Immunohistochemical Analysis of mTOR Pathway-Related Proteins in Kaposiform Hemangioendothelioma.
Background: Mammalian target of rapamycin (mTOR) inhibitors have been shown to have excellent effects in the management of kaposiform hemangioendothelioma (KHE); however, the mechanism of action is unclear. This study identified the expressions of mTOR pathway-related proteins in different vascular...
| Publicado en: | Dermatology (10188665) Vol. 236; no. 3; pp. 262 - 271 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Karger AG
2020
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143156178&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143156178 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10188665 NIG jtl: Dermatology (10188665) issn: 10188665 maglogo: N pubinfo: dt: 2020 vid: 236 iid: 3 pid: 2485 pub: Karger AG artinfo: ui: 143156178 143156178 146764506 NLM31896113 143156178 10.1159/000503604 NLM31896113 143156178 ppf: 262 ppct: 9 formats: tig: atl: Immunohistochemical Analysis of mTOR Pathway-Related Proteins in Kaposiform Hemangioendothelioma. aug: au: Wang, Zuopeng Zheng, Chao Sun, Hongqiang Yao, Wei Li, Kai Ma, Yangyang Zheng, Shan affil: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China sug: subj: Immunohistochemistry Methods Kasabach-Merritt Syndrome Metabolism Hemangioma Metabolism Sarcoma, Kaposi's Metabolism Sirolimus Therapeutic Use Signal Transduction Antineoplastic Agents Therapeutic Use Kasabach-Merritt Syndrome Drug Therapy Lymphatic Abnormalities Metabolism Hemangioma Drug Therapy Epithelial Cells Metabolism Retrospective Design Sarcoma, Kaposi's Drug Therapy Human ab: Background: Mammalian target of rapamycin (mTOR) inhibitors have been shown to have excellent effects in the management of kaposiform hemangioendothelioma (KHE); however, the mechanism of action is unclear. This study identified the expressions of mTOR pathway-related proteins in different vascular tumors to provide insight into the pathogenesis of KHE.Methods: We retrospectively reviewed the pathologic specimens of 30 patients (KHE, 15; tufted angioma [TA], 5; infantile hemangioma [IH], 5; and lymphatic malformation [LM], 5). The immunohistochemical expression of mTOR-related proteins tuberous sclerosis complex 2 (TSC2), phosphatase and tensin homologue (PTEN), phosphorylated eukaryotic translation initiation factor 4E binding protein 1 (p-4EBP1), phosphorylated mTOR (p-mTOR), and phosphorylated ribosomal protein S6 kinase B1 (p-P70S6K) were analyzed using Image-Pro Plus software. KHE had the following pattern of expression in the spindle vascular endothelial cells: TSC2 (-); PTEN (-); p-4EBP1 (+); p-mTOR (+); and p-P70S6K (+).Results: All 3 patients treated with sirolimus had good responses. The TA results were similar to KHE with no significant differences (p-4EBP1: p = 0.0687; p-mTOR: p = 0.0832). The expressions of TSC2, PTEN, p-4EBP1, p-mTOR, and p-P70S6K were negative or weakly positive in IH with a statistically significant difference compared to KHE (p-4EBP1: p < 0.001; p-mTOR: p < 0.001; p-P70S6K: p < 0.001). LM had no significant differences when compared to KHE.Conclusions: The absence of TSC2 and PTEN caused abnormal activation of the mTOR signaling pathway and may be involved in the pathogenesis of KHE. The expression of mTOR-related proteins in TA and LM was similar to KHE, unlike IH. The KHE pattern of expression [PTEN (-), TSC2 (-), p-mTOR (+), p-P70S6K (+), and p-4EBP1 (+)] suggested that sirolimus may be a good therapeutic choice. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|