Targeted proteomic response to coffee consumption.
Purpose: Coffee is widely consumed and implicated in numerous health outcomes but the mechanisms by which coffee contributes to health is unclear. The purpose of this study was to test the effect of coffee drinking on candidate proteins involved in cardiovascular, immuno-oncological and neurological...
| Published in: | European Journal of Nutrition Vol. 59; no. 4; pp. 1529 - 1540 |
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| Main Authors: | , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Springer Nature
Jun2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143245181&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143245181 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Jun2020 vid: 59 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 143245181 143245181 143245181 10.1007/s00394-019-02009-1 143245181 ppf: 1529 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Targeted proteomic response to coffee consumption. aug: au: Kuang, Alan Erlund, Iris Herder, Christian Westerhuis, Johan A. Tuomilehto, Jaakko Cornelis, Marilyn C. affil: Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, 680 North Lake Shore Drive, Suite 1400, 60611, Chicago, IL, USA sug: subj: Coffee Metabolism Food Intake Proteomics Peptides Metabolism Biological Markers Metabolism Coffee Administration and Dosage Human Fasting Quality Control (Technology) Repeated Measures Analysis of Variance Immunoassay Methods Peptide Hydrolases Metabolism Neutral Ceramidase Metabolism Clinical Trials Cardiovascular System Metabolism Immune System Metabolism Nervous System Metabolism Signal Transduction ab: Purpose: Coffee is widely consumed and implicated in numerous health outcomes but the mechanisms by which coffee contributes to health is unclear. The purpose of this study was to test the effect of coffee drinking on candidate proteins involved in cardiovascular, immuno-oncological and neurological pathways. Methods: We examined fasting serum samples collected from a previously reported single blinded, three-stage clinical trial. Forty-seven habitual coffee consumers refrained from drinking coffee for 1 month, consumed 4 cups of coffee/day in the second month and 8 cups/day in the third month. Samples collected after each coffee stage were analyzed using three multiplex proximity extension assays that, after quality control, measured a total of 247 proteins implicated in cardiovascular, immuno-oncological and neurological pathways and of which 59 were previously linked to coffee exposure. Repeated measures ANOVA was used to test the relationship between coffee treatment and each protein. Results: Two neurology-related proteins including carboxypeptidase M (CPM) and neutral ceramidase (N-CDase or ASAH2), significantly increased after coffee intake (P < 0.05 and Q < 0.05). An additional 46 proteins were nominally associated with coffee intake (P < 0.05 and Q > 0.05); 9, 8 and 29 of these proteins related to cardiovascular, immuno-oncological and neurological pathways, respectively, and the levels of 41 increased with coffee intake. Conclusions: CPM and N-CDase levels increased in response to coffee intake. These proteins have not previously been linked to coffee and are thus novel markers of coffee response worthy of further study. Clinical trial registry: http://www.isrctn.com/ISRCTN12547806. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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