An immune gene expression signature distinguishes central nervous system metastases from primary tumours in non–small-cell lung cancer.
Dissemination of non–small-cell lung cancer (NSCLC) in the central nervous system is a frequent and challenging clinical problem. Systemic or local therapies rarely prolong survival and have modest activity regarding local control. Alterations in gene expression in brain metastasis versus primary tu...
| Publicado en: | European Journal of Cancer Vol. 132; pp. 24 - 35 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Pergamon Press - An Imprint of Elsevier Science
Jun2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143363430&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143363430 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09598049 1VW jtl: European Journal of Cancer issn: 09598049 maglogo: N pubinfo: dt: Jun2020 vid: 132 pid: 2410 pub: Pergamon Press - An Imprint of Elsevier Science artinfo: ui: 143363430 143363430 143363430 10.1016/j.ejca.2020.03.014 143363430 ppf: 24 ppct: 11 formats: tig: atl: An immune gene expression signature distinguishes central nervous system metastases from primary tumours in non–small-cell lung cancer. aug: au: Tsakonas, Georgios Lewensohn, Rolf Botling, Johan Ortiz-Villalon, Cristian Micke, Patrick Friesland, Signe Nord, Helena Lindskog, Magnus Sandelin, Martin Hydbring, Per Ekman, Simon affil: Thoracic Oncology Center, Karolinska University Hospital, Stockholm, Sweden sug: subj: Gene Expression Profiling Neoplasm Metastasis Brain Neoplasms Immunology Carcinoma, Non-Small-Cell Lung Immunology Brain Neoplasms Familial and Genetic Carcinoma, Non-Small-Cell Lung Familial and Genetic Brain Neoplasms Surgery Carcinoma, Non-Small-Cell Lung Surgery Human Prospective Studies Data Analysis Software Treatment Outcomes ab: Dissemination of non–small-cell lung cancer (NSCLC) in the central nervous system is a frequent and challenging clinical problem. Systemic or local therapies rarely prolong survival and have modest activity regarding local control. Alterations in gene expression in brain metastasis versus primary tumour may increase aggressiveness and impair therapeutic efforts. We identified 25 patients with surgically removed NSCLC brain metastases in two different patient cohorts. For 13 of these patients, primary tumour samples were available. Gene expression analysis using the nCounter® PanCancer Immune Profiling gene expression panel (nanoString technologies Inc.) was performed in brain metastases and primary tumour samples. Identification of differentially expressed genes was conducted on normalized data using the nSolver analysis software. We compared gene expression patterns in brain metastases with primary tumours. Brain metastasis samples displayed a distinct clustering pattern compared to primary tumour samples with a statistically significant downregulation of genes related to immune response and immune cell activation. Results from KEGG term analysis on differentially expressed genes revealed a concomitant enrichment of multiple KEGG terms associated with the immune system. We identified a 12-gene immune signature that clearly separated brain metastases from primary tumours. We identified a unique gene downregulation pattern in brain metastases compared with primary tumours. This finding may explain the lower intracranial efficacy of systemic therapy, especially immunotherapy, in brain metastasis of patients with NSCLC. • Gene expression matched analysis was performed in primary vs brain metastatic non–small-cell lung cancer (NSCLC). • We identified a unique immune gene downregulation pattern in brain metastatic NSCLC. • A diagnostic 12-gene signature for brain metastatic NSCLC was identified. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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