Antifungal activity of eugenol on Cryptococcus neoformans biological activity and Cxt1p gene expression.
Background and Purpose: The present study was targeted toward investigating the effects of eugenol on Cryptococcus neoformans biological activity and Cxt1p gene expression. Materials and Methods: For the purpose of the study, the growth, urease, synergism activity, and disk diffusion of C. neoforman...
| Publicado en: | Current Medical Mycology Vol. 6; no. 1; pp. 9 - 15 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Mazandaran University of Medical Sciences
Mar2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143374466&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143374466 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 24233439 JKN0 jtl: Current Medical Mycology issn: 24233439 maglogo: N pubinfo: dt: Mar2020 vid: 6 iid: 1 pid: 45683 pub: Mazandaran University of Medical Sciences artinfo: ui: 143374466 143374466 143374466 10.18502/cmm.6.1.2502 143374466 ppf: 9 ppct: 6 formats: tig: atl: Antifungal activity of eugenol on Cryptococcus neoformans biological activity and Cxt1p gene expression. aug: au: Hassanpour, Parviz Shams-Ghahfarokhi, Masoomeh Razzaghi-Abyaneh, Mehdi affil: Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran sug: subj: Antifungal Agents Pharmacodynamics Cryptococcus Drug Effects Gene Expression Drug Effects Acids, Carbocyclic Pharmacodynamics Human Urease Drug Synergism Polymerase Chain Reaction Fluconazole Growth Inhibitors ab: Background and Purpose: The present study was targeted toward investigating the effects of eugenol on Cryptococcus neoformans biological activity and Cxt1p gene expression. Materials and Methods: For the purpose of the study, the growth, urease, synergism activity, and disk diffusion of C. neoformans were assessed in eugenol-treated culture. The minimum inhibitory concentration (MIC) was determined by the Clinical and Laboratory Standards Institute M27-A3 method at a concentration range of 0.062-2 mg/mL. Subsequently, the expression of Cxt1p genes was studied at the MIC50 concentration of eugenol using real-time polymerase chain reaction. Results: The obtained results showed that eugenol at the concentrations of 125 and 500 µg/mL resulted in 50% and 100% growth inhibition in C. neoformans, respectively. In terms of urease activity, the results showed that the addition of MIC50 of eugenol and fluconazole to urea medium reduced urease activity in C. neoformans. In the culture treated with eugenol, the inhibition zone of antifungal drugs, namely amphotericin B, itraconazole, and fluconazole, was increased to 36±0.002, 22±0.001, and 12±0.002 mm, respectively. The expression levels of Cxt1p in the eugenol-treated, fluconazole-treated, and non-treated samples were estimated at 46%, 58%, and 100%, respectively. Conclusion: The findings of the current study revealed that eugenol could cause C. neoformans growth inhibition and reduce Cxt1p expression in this species. As the results indicated, the susceptibility of C. neoformans to fluconazole was increased when combined with eugenol. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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