SPECT Imaging of Muscle Injury with [99mTc]MDP in a Mouse Model of Muscular Dystrophy.

Purpose: Tc-99m methylene diphosphonate ([99mTc]MDP) is an in vivo bone imaging agent that also accumulates in injured skeletal muscle cells. The objective of this study was to investigate if [99mTc]MDP could be used to detect muscle injury in the mdx mouse model of Duchenne muscular dystrophy (DMD)...

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Publicado en:Molecular Imaging & Biology Vol. 22; no. 3; pp. 562 - 569
Autores principales: Gaut, Carolynn, Sullivan, Jenna M., Biscaro, Barbara, Soares, Edward J., Nicholson, Katharine, Hoppin, Jack, Verma, Ajay
Formato: research Journal Article
Publicado: Springer Nature Jun2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2020
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      pub: Springer Nature
      place: New York, New York
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        atl: SPECT Imaging of Muscle Injury with [99mTc]MDP in a Mouse Model of Muscular Dystrophy.
      aug:
        au:
          Gaut, Carolynn
          Sullivan, Jenna M.
          Biscaro, Barbara
          Soares, Edward J.
          Nicholson, Katharine
          Hoppin, Jack
          Verma, Ajay
        affil: Invicro, Boston, MA, USA
      sug:
        subj:
          Muscular Dystrophy
          Muscles
          Radiopharmaceuticals Pharmacokinetics
          Muscles Injuries
          Muscles Metabolism
          Mice
          Muscular Dystrophy Pathology
          Models, Biological
          Muscular Dystrophy Metabolism
          Muscles Pathology
          Male
          Diagnostic Imaging Methods
          Animal Studies
          Radiopharmaceuticals
          Comparative Studies
          Multicenter Studies
          Evaluation Research
          Validation Studies
          Male
      ab: Purpose: Tc-99m methylene diphosphonate ([99mTc]MDP) is an in vivo bone imaging agent that also accumulates in injured skeletal muscle cells. The objective of this study was to investigate if [99mTc]MDP could be used to detect muscle injury in the mdx mouse model of Duchenne muscular dystrophy (DMD).Procedures: Static whole-body single-photon emission computed tomography/computed tomography (CT) scans were acquired at 2 h post-injection of [99mTc]MDP in two cohorts of animals at different sites: one cohort of mice at 6, 15, and 19 weeks of age, and a separate cohort at 16 weeks. The second cohort was also imaged with high-resolution CT at 8 weeks.Results: mdx mice had higher [99mTc]MDP uptake and significantly higher [99mTc]MDP concentrations in muscle than controls.Conclusions: Higher uptake of [99mTc]MDP in muscle of mdx mice agrees with histological reports of muscle calcification in mdx mice, and suggests the potential translational use of [99mTc]MDP imaging for tracking DMD progression and therapeutic response.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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