Paramagnetic Fluorinated Nanoemulsions for in vivo F-19 MRI.
Purpose: We aim to develop perfluorocarbon-based nanoemulsions with improved sensitivity for detection of inflammatory macrophages in situ using F-19 MRI. Towards this goal, we evaluate the feasibility of nanoemulsion formulation incorporating a metal chelate in the fluorous phase which shortens the...
| Publicado en: | Molecular Imaging & Biology Vol. 22; no. 3; pp. 665 - 675 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jun2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143439707&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143439707 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2020 vid: 22 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 143439707 143439707 NLM31482414 143439707 10.1007/s11307-019-01415-5 NLM31482414 143439707 ppf: 665 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Paramagnetic Fluorinated Nanoemulsions for in vivo F-19 MRI. aug: au: Rho, Junsung Stares, Emma Adams, Stephen R. Lister, Deanne Leach, Benjamin Ahrens, Eric T. affil: Department of Radiology, University of California, San Diego, 9500 Gilman Dr. #0695, 92093-0695, La Jolla, CA, USA sug: subj: Macrophages Magnetic Resonance Imaging Methods Fluorocarbons Inflammation Nanostructures Models, Biological Ferric Compounds Animal Studies Cell Line Inflammation Pathology Emulsions Polymers Female Inflammation Immunology Mice Macrophages Immunology Comparative Studies Multicenter Studies Evaluation Research Validation Studies Female ab: Purpose: We aim to develop perfluorocarbon-based nanoemulsions with improved sensitivity for detection of inflammatory macrophages in situ using F-19 MRI. Towards this goal, we evaluate the feasibility of nanoemulsion formulation incorporating a metal chelate in the fluorous phase which shortens the F-19 longitudinal relaxation rate and image acquisition time.Procedures: Perfluorinated linear polymers were conjugated to metal-binding tris-diketonate, blended with unconjugated polymers, and emulsified in water. Phospholipid-based surfactant was used to stabilize nanoemulsion and provide biocompatibility. Nanoemulsions were metalated with the addition of ferric salt to the buffer. Physical stability of surfactant and nanoemulsion was evaluated by mass spectrometry and dynamic light scattering measurements. Nanoemulsions were injected intravenously into a murine granuloma inflammation model, and in vivo19F/1H MRI at 11.7 T was performed.Results: We demonstrated stability and biocompatibility of lipid-based paramagnetic nanoemulsions. We investigated potential oxidation of lipid in the presence of metal chelate. As a proof of concept, we performed non-invasive monitoring of macrophage burden in a murine inflammation model following intravenous injection of nanoemulsion using in vivo F-19 MRI.Conclusion: Lipid-based nanoemulsion probes of perfluorocarbon synthesized with iron-binding fluorinated β-diketones can be formulated for intravenous delivery and inflammation detection in vivo. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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