SSH: A Tool for Predicting Hydrophobic Interaction of Monoclonal Antibodies Using Sequences.

Therapeutic antibodies are one of the most important parts of the pharmaceutical industry. They are widely used in treating various diseases such as autoimmune diseases, cancer, inflammation, and infectious diseases. Their development process however is often brought to a standstill or takes a longe...

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Publicado en:BioMed Research International pp. 1 - 7
Autores principales: Dzisoo, Anthony Mackitz, Kang, Juanjuan, Yao, Pengcheng, Klugah-Brown, Benjamin, Mengesha, Birga Anteneh, Huang, Jian
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 6/2/2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 6/2/2020
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2020/3508107
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        atl: SSH: A Tool for Predicting Hydrophobic Interaction of Monoclonal Antibodies Using Sequences.
      aug:
        au:
          Dzisoo, Anthony Mackitz
          Kang, Juanjuan
          Yao, Pengcheng
          Klugah-Brown, Benjamin
          Mengesha, Birga Anteneh
          Huang, Jian
        affil: Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 611731, China
      sug:
        subj:
          Chemistry, Physical
          Antibodies, Monoclonal Analysis
          Sequence Analysis Methods
          Drug Design
          Human
          Chromatography Methods
          Spectrum Analysis Methods
          Sensitivity and Specificity
          Correlation Coefficient
          ROC Curve
      ab: Therapeutic antibodies are one of the most important parts of the pharmaceutical industry. They are widely used in treating various diseases such as autoimmune diseases, cancer, inflammation, and infectious diseases. Their development process however is often brought to a standstill or takes a longer time and is then more expensive due to their hydrophobicity problems. Hydrophobic interactions can cause problems on half-life, drug administration, and immunogenicity at all stages of antibody drug development. Some of the most widely accepted and used technologies for determining the hydrophobic interactions of antibodies include standup monolayer adsorption chromatography (SMAC), salt-gradient affinity-capture self-interaction nanoparticle spectroscopy (SGAC-SINS), and hydrophobic interaction chromatography (HIC). However, to measure SMAC, SGAC-SINS, and HIC for hundreds of antibody drug candidates is time-consuming and costly. To save time and money, a predictor called SSH is developed. Based on the antibody's sequence only, it can predict the hydrophobic interactions of monoclonal antibodies (mAbs). Using the leave-one-out crossvalidation, SSH achieved 91.226% accuracy, 96.396% sensitivity or recall, 84.196% specificity, 87.754% precision, 0.828 Mathew correlation coefficient (MCC), 0.919 f -score, and 0.961 area under the receiver operating characteristic (ROC) curve (AUC).
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
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        Journal Article
      ougenre: Article
    language: English
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