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CX3CL1/CX3CR1-signalling in the CD9/S100β/SOX2-positive adult pituitary stem/progenitor cells modulates differentiation into endothelial cells.

Approximately 8% of CD9-, S100β- and SOX2-triple positive (CD9/S100β/SOX2-positive) stem/progenitor cells in the anterior lobe of the rat pituitary gland have previously been shown to differentiate into endothelial cells in vitro, suggesting that they play a role in vascularisation as tissue-residen...

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Published in:Histochemistry & Cell Biology Vol. 153; no. 6; pp. 385 - 397
Main Authors: Horiguchi, Kotaro, Fujiwara, Ken, Yoshida, Saishu, Tsukada, Takehiro, Hasegawa, Rumi, Takigami, Shu, Ohsako, Shunji, Yashiro, Takashi, Kato, Takako, Kato, Yukio
Format: Journal Article
Published: Springer Nature Jun2020
Online Access:View this record in EBSCOhost
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      jtl: Histochemistry & Cell Biology
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      dt: Jun2020
      vid: 153
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00418-020-01862-0
        143819579
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        atl: CX3CL1/CX3CR1-signalling in the CD9/S100β/SOX2-positive adult pituitary stem/progenitor cells modulates differentiation into endothelial cells.
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        au:
          Horiguchi, Kotaro
          Fujiwara, Ken
          Yoshida, Saishu
          Tsukada, Takehiro
          Hasegawa, Rumi
          Takigami, Shu
          Ohsako, Shunji
          Yashiro, Takashi
          Kato, Takako
          Kato, Yukio
        affil: Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, 181-8612, Mitaka, Tokyo, Japan
      sug:
      ab: Approximately 8% of CD9-, S100β- and SOX2-triple positive (CD9/S100β/SOX2-positive) stem/progenitor cells in the anterior lobe of the rat pituitary gland have previously been shown to differentiate into endothelial cells in vitro, suggesting that they play a role in vascularisation as tissue-resident vascular precursor cells. In the present study, we focused on chemokine ligands to further characterise the CD9/S100β/SOX2-positive cells and found that they distinctively express CX3C chemokine ligand 1 (Cx3cl1). Immunohistochemical analysis of the anterior lobe showed that CX3CL1-positive cells comprised 7.8% in CD9-positive cells. By cultivation of the CD9-positive cells on laminin-coated plates, we observed that the expression levels of Cx3cl1 decreased, while those of Sox18, an endothelial cell-progenitor marker, and Cx3cr1, a CX3CL1 receptor, increased. Furthermore, in a rat model of prolactinoma, the most common pituitary tumour, which is accompanied by frequent neo-vasculogenesis in the anterior lobe, we have confirmed a decrease in Cx3cl1 expression and an increase in Cx3cr1 expression, as well as a prominent increase in Sox18 expression. These findings suggest that CX3CL1/CX3CR1 signalling in CD9/S100β/SOX2-positive cells plays an important role in resupplying endothelial cells for vascular remodelling in the anterior lobe.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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