Nanoscale imaging using differential expansion microscopy.
Expensive and time-consuming approaches of immunoelectron microscopy of biopsy tissues continues to serve as the gold-standard for diagnostic pathology. The recent development of the new approach of expansion microscopy (ExM) capable of fourfold lateral expansion of biological specimens for their mo...
| Publicado en: | Histochemistry & Cell Biology Vol. 153; no. 6; pp. 469 - 481 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jun2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143819581&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143819581 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Jun2020 vid: 153 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 143819581 143998247 10.1007/s00418-020-01869-7 143819581 ppf: 469 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Nanoscale imaging using differential expansion microscopy. aug: au: Pernal, Sebastian P. Liyanaarachchi, Asiri Gatti, Domenico L. Formosa, Brent Pulvender, Rishika Kuhn, Eric R. Ramos, Rafael Naik, Akshata R. George, Kathleen Arslanturk, Suzan Taatjes, Douglas J. Jena, Bhanu P. affil: Department of Physiology, Wayne State University School of Medicine, 540 E. Canfield, 5245, 5215 Scott Hall, 48201, Detroit, MI, USA sug: ab: Expensive and time-consuming approaches of immunoelectron microscopy of biopsy tissues continues to serve as the gold-standard for diagnostic pathology. The recent development of the new approach of expansion microscopy (ExM) capable of fourfold lateral expansion of biological specimens for their morphological examination at approximately 70 nm lateral resolution using ordinary diffraction limited optical microscopy, is a major advancement in cellular imaging. Here we report (1) an optimized fixation protocol for retention of cellular morphology while obtaining optimal expansion, (2) an ExM procedure for up to eightfold lateral and over 500-fold volumetric expansion, (3) demonstrate that ExM is anisotropic or differential between tissues, cellular organelles and domains within organelles themselves, and (4) apply image analysis and machine learning (ML) approaches to precisely assess differentially expanded cellular structures. We refer to this enhanced ExM approach combined with ML as differential expansion microscopy (DiExM), applicable to profiling biological specimens at the nanometer scale. DiExM holds great promise for the precise, rapid and inexpensive diagnosis of disease from pathological specimen slides. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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