Low myocardial transcript variant alt-a of cyclin dependent kinase inhibitor p21 expression differentiates hypothermia from cardiac/respiratory causes of death.

Gene expressions in the myocardium have been shown to vary between different causes of death, which can be utilized in the recognition of varied processes. Our previous work with a limited number of cases showed a high messenger ribonucleic acid expression of the transcript variant alt-a of cyclin d...

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Publicado en:Medicine Vol. 99; no. 9; pp. 1 - 6
Autores principales: Kaija, Helena, Pakanen, Lasse, Porvari, Katja
Formato: Journal Article
Publicado: Lippincott Williams & Wilkins Feb2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Feb2020
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      pub: Lippincott Williams & Wilkins
      place: Baltimore, Maryland
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        atl: Low myocardial transcript variant alt-a of cyclin dependent kinase inhibitor p21 expression differentiates hypothermia from cardiac/respiratory causes of death.
      aug:
        au:
          Kaija, Helena
          Pakanen, Lasse
          Porvari, Katja
        affil: Department of Forensic Medicine, Research Unit of Internal Medicine, Faculty of Medicine, Medical Research Center Oulu, Oulu University Hospital and University of Oulu
      sug:
        subj:
          Cause of Death
          Hypothermia Physiopathology
          Heart Diseases Physiopathology
          Myocardium Pathology
          Proteins Analysis
          Hypothermia Pathology
          Finland
          Heart Diseases Pathology
          Ferrans and Powers Quality of Life Index
          Scales
      ab: Gene expressions in the myocardium have been shown to vary between different causes of death, which can be utilized in the recognition of varied processes. Our previous work with a limited number of cases showed a high messenger ribonucleic acid expression of the transcript variant alt-a of cyclin dependent kinase inhibitor p21 (p21 alt-a) in chronic cardiac ischemia deaths and a low expression in hypothermia deaths and acute myocardial ischemia deaths. In present work, p21 alt-a expression in the myocardium of human cadavers was calculated using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. In this collection of 143 samples, the p21 alt-a expression was significantly lower in hypothermia than in chronic cardiac ischemic heart disease with (P < .001) or without (P < .001) acute myocardial infarction and in other cardiac and respiratory disease deaths (P < .000). Chronic ischemic heart disease in hypothermia cases did not increase the expression. The p21 alt-a expression did not correlate with postmortem interval, quality of RNA or with the age of the deceased. The p21 alt-a referenced to GAPDH expression in cadaver myocardium has apparent potential as a marker distinguishing between hypothermia and cardiac/respiratory diseases as causes of death.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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