Association between PPAR γ rs1801282 polymorphism with diabetic nephropathy and type-2 diabetes mellitus.
Background: Diabetic Nephropathy (DN) is a major complication of Type 2 Diabetes Mellitus (T2DM) with high morbidity rates worldwide. Objective: To determine the association of PPARγ rs1801282 polymorphism in T2DM and DN in south Indian population. Methods: We have conducted a case-control study to...
| Publicado en: | Nefrología Vol. 40; no. 3; pp. 287 - 299 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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Revista Nefrologia
May/Jun2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=144216003&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 144216003 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 02116995 50C3 jtl: Nefrología issn: 02116995 maglogo: N pubinfo: dt: May/Jun2020 vid: 40 iid: 3 pid: 39358 pub: Revista Nefrologia artinfo: ui: 144216003 10.1016/j.nefro.2020.01.005 ppf: 287 ppct: 12 formats: fmt: @attributes: type: P size: 2.9MB tig: atl: Association between PPAR γ rs1801282 polymorphism with diabetic nephropathy and type-2 diabetes mellitus. aug: au: Regine, Ilibagiza Akram Husain, Rehman Syed Rasheed Aswathi, Rajagopalan P. Reddy, D. Ramacharan Ahmed, Shiek S. S. J. Ramakrishnan, Veerabathiran affil: Genetics Lab, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu, India Department of General Medicine, Chettinad Hospital and Research Institute, Chettinad Health City, Kelambakkam, Tamil Nadu, India Drug Discovery Lab, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu, India su: Diabetes Type 2 diabetes Peroxisome proliferator-activated receptors Diabetic nephropathies Genetic models sug: subj: Diabetes Type 2 diabetes Peroxisome proliferator-activated receptors Diabetic nephropathies Genetic models keyword: ARMS-PCR Diabetic Nephropathy Modelos genéticos Nefropatía diabética PNU PPARγ RCP-ARMS SNP Modelos genéticos Nefropatía diabética PNU RCP-ARMS ab: Background: Diabetic Nephropathy (DN) is a major complication of Type 2 Diabetes Mellitus (T2DM) with high morbidity rates worldwide. Objective: To determine the association of PPARγ rs1801282 polymorphism in T2DM and DN in south Indian population. Methods: We have conducted a case-control study to test the association of rs1801282 polymorphism with T2DM and DN in 424 subjects (DN = 128; T2DM = 148 and controls = 148) belonging to the south Indian population using ARMS-PCR and Sanger sequencing method. Further, a meta-analysis was performed for rs1801282 polymorphism from the published literature retrieved from various electronic databases to determine the susceptibility among T2DM and DN across various ethnic populations under five genetic models. Results: The genotyping of rs1801282 polymorphism showed significant (p-value < 0.05) association with DN and T2DM compared to controls. In the meta-analysis, no significant association (p-value > 0.05) was noticed for rs1801282 with DN vs. controls in homozygote, heterozygote, allelic, recessive and dominant genetic models. However, a significant association was observed between rs1801282 SNP and T2DM under heterozygote (Jj vs JJ) genetic model with OR = 0.56, (95%CI [0.43-0.74]), p = 0.0001 of Asian and Caucasian populations. Conclusion: Overall analysis suggests that the rs1801282 polymorphism might be associated with DN and T2DM. More case-control studies on the PPARγ gene with a larger sample size including all the confounding factors are required to corroborate the findings from this meta-analysis. Antecedentes: La nefropatía diabética (ND) es una complicación importante de la diabetes mellitus de tipo 2 (DMT2) con altas tasas de morbilidad mundial. Objetivo: Determinar la asociación del polimorfismo rs1801282 de PPARγ en la DMT2 y la ND en la población del sur de India. Métodos: Hemos llevado a cabo un estudio de casos y controles para analizar la asociación del polimorfismo rs1801282 con la DMT2 y la ND en 424 sujetos (ND = 128; DMT2 = 148 y controles = 148) pertenecientes a la población del sur de India mediante RCP-ARMS y método de secuenciación de Sanger. Además, se realizó un metaanálisis para el polimorfismo de rs1801282 a partir de la literatura publicada en varias bases de datos electrónicas para determinar la sensibilidad entre la DMT2 y la ND en varias poblaciones étnicas con 5 modelos genéticos. Resultados: El genotipado de polimorfismo rs1801282 demostró una asociación significativa (valor de p < 0,05) con la ND y la DMT2 en comparación con los controles. En el metaanálisis no se observó asociación significativa (valor de p > 0,05) de rs1801282 con la ND frente a los controles en modelos genéticos homocigóticos, heterocigóticos, alélicos, recesivos y dominantes. Sin embargo, se observó una asociación significativa entre el polimorfismo de nucleótido único (PNU) rs1801282 SNP y la DMT2 en el modelo genético heterocigótico (Jj frente a JJ) con OR = 0,56, (IC del 95%: 0,43-0,74; p = 0,0001 de poblaciones asiáticas y caucásicas. Conclusión: El análisis general sugiere que el polimorfismo rs1801282 puede asociarse a ND y a DMT2. Se precisan más estudios de casos y controles sobre el gen PPARγ con un tamaño de la muestra mayor que incluya todos los factores de confusión para corroborar los resultados de este metaanálisis. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Nefrología is the property of Revista Nefrologia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Nefrología holder: Revista Nefrologia dt: @attributes: year: 2020 holdings: @attributes: islocal: N |
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