Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in db/db Mice.
Numerous studies have reported that autophagy plays an important role in chronic wound healing, and enhancement of autophagic activity impairs cutaneous wound healing. The autophagy inhibitor Bafilomycin A1 (Baf A1) inhibits autophagy by preventing the formation of autophagosomes. This study aimed a...
| Publicado en: | BioMed Research International pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
7/3/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=144375006&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 144375006 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/3/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 144375006 144375006 144375006 10.1155/2020/6265701 144375006 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in db/db Mice. aug: au: Wang, Fan Zhang, Chao Dai, Linna Zhang, Yulu Wang, Yongxue Hao, Yongwei Ji, Shenglu Xu, Zhihao Han, Na Chen, Hongli Zhang, Qiqing Nan, Wenbin affil: Life Science and Health Research Institute, College of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003 Henan, China sug: subj: Antibiotics, Macrolide Pharmacodynamics Blood Glucose Drug Effects Diabetes Mellitus Drug Therapy Wound Healing Animal Studies Mice Autophagy Drug Effects Epidermis Drug Effects Energy Metabolism Drug Effects Antigens Drug Effects Gene Expression Drug Effects Immunohistochemistry Collagen Drug Effects Staining and Labeling Methods Cytokines Drug Effects Tumor Necrosis Factor Drug Effects Cell Proliferation Drug Effects ab: Numerous studies have reported that autophagy plays an important role in chronic wound healing, and enhancement of autophagic activity impairs cutaneous wound healing. The autophagy inhibitor Bafilomycin A1 (Baf A1) inhibits autophagy by preventing the formation of autophagosomes. This study aimed at elucidating the effect of Bafilomycin A1 on chronic refractory wound healing in diabetic mice. A total of 40 diabetic (db/db) mice and 20 nondiabetic (db/m) mice were used in this study. Full-thickness skin defects were generated in the db/db mice models, which were then divided into the following two groups: the nontreated (db/db group) and Baf A1-treated groups (Baf A1 group). The same skin defects were generated in db/m mice (db/m group) to serve as a control. We demonstrated that Baf A1 treatment significantly accelerated wound healing in db/db mice and exerted good healing effects. Moreover, Baf A1 inhibited autophagy in the newly generated epidermis and had minor effects on metabolism in db/db mice. PCNA expression, as detected by immunohistochemistry, and collagen thickness, as detected by Masson's trichrome staining on the 14th day, were higher in the db/m and Baf A1 groups than in the db/db group. In addition, the expression of the proinflammatory cytokine TNF-α in the db/m and Baf A1 groups increased significantly on day 6, and the expression of the anti-inflammatory cytokine IL-10 also increased significantly on day 9. However, there were no significant changes in the expression levels of TNF-α and IL-10 in the db/db group. Therefore, Baf A1 may accelerate diabetic chronic refractory wound healing by promoting cell proliferation, collagen production, and regulating the inflammatory balance. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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