Pre-meal protein intake alters postprandial plasma metabolome in subjects with metabolic syndrome.

Purpose: We examined the effect on the postprandial plasma metabolome of protein pre-meals before a fat-rich main meal. Methods: Two randomized, cross-over meal studies were conducted to test the dose–response effect (0 g, 10 g, 20 g) of a pre-meal with whey protein (WP) (PREMEAL I), and the effect...

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Publicado en:European Journal of Nutrition Vol. 59; no. 5; pp. 1881 - 1895
Autores principales: Pekmez, Ceyda Tugba, Bjørnshave, Ann, Pratico, Giulia, Hermansen, Kjeld, Dragsted, Lars Ove
Formato: research tables/charts randomized controlled trial Journal Article
Publicado: Springer Nature Aug2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Aug2020
      vid: 59
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00394-019-02039-9
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        atl: Pre-meal protein intake alters postprandial plasma metabolome in subjects with metabolic syndrome.
      aug:
        au:
          Pekmez, Ceyda Tugba
          Bjørnshave, Ann
          Pratico, Giulia
          Hermansen, Kjeld
          Dragsted, Lars Ove
        affil: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark
      sug:
        subj:
          Postprandial Period
          Metabolomics
          Metabolic Syndrome X
          Food Intake
          Proteins
          Meals
          Randomized Controlled Trials
          Crossover Design
          Diet, Atherogenic
          Chromatography, Liquid
          Mass Spectrometry
          Gluten
          Caseins
          Amino Acids, Branched-Chain
          Whey Proteins
          Breakfast
          Metabolites
          Insulin Resistance
          Biological Markers
          Human
      ab: Purpose: We examined the effect on the postprandial plasma metabolome of protein pre-meals before a fat-rich main meal. Methods: Two randomized, cross-over meal studies were conducted to test the dose–response effect (0 g, 10 g, 20 g) of a pre-meal with whey protein (WP) (PREMEAL I), and the effect of protein quality (10 g WP, casein, or gluten) and timing (− 15 min vs − 30 min) of the pre-meal (PREMEAL II). Participants with metabolic syndrome received one of the test meals on each test day, − 15 min (or − 30 min) prior to a standardized fat-rich breakfast. Plasma samples were collected at − 15 min (or − 30 min), 0, 120, 240 a nd 360 min and analyzed using liquid chromatography–mass spectrometry with an untargeted method. Results: Pre-meal WP intake elevated plasma branched-chain amino acids (BCAA), aromatic amino acids and methionine and decreased plasma LPC (16:0) and PC (32:1) levels before the main meal. Early (− 15 to 0 min) aromatic amino acids and BCAA in response to pre-meal WP partially predict the glucose and insulin response after the main meal. A pre-meal with WP altered the postprandial plasma metabolic pattern of acyl-carnitines, specific PCs, LPCs and LPEs, betaine, citric acid, linoleic acid, and β-hydroxypalmitic acid compared to no pre-meal. The casein and WP pre-meals exhibited similar postprandial amino acid responses whereas a pre-meal with gluten resulted in lower levels of plasma amino acids and its metabolites. Conclusion: A pre-meal with protein affects the postprandial metabolic pattern indicating facilitated glucose and lipid disposal from plasma in participants with metabolic syndrome.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        randomized controlled trial
        Journal Article
      ougenre: Article
    language: English
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