anti-CD25 CAR مرتبط با qRT-PCR و RNA تجزیه وتحلیل های ساختار ثانویه NK- بیان شده در رده ی سلولی 92
Background and Objectives: Tumor-infiltrating regulatory T (TI-Treg) cells perform an important function in cancer immune escape. In this study, the third generation CAR construct was designed against human CD25 antigen as the cell surface biomarker of TI-Treg cells. Methods: At first, the construct...
| Publicado en: | Qom University of Medical Sciences Journal Vol. 14; no. 2; pp. 1 - 13 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Qom University of Medical Sciences
Apr2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=144695606&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 144695606 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17357799 8P2V jtl: Qom University of Medical Sciences Journal issn: 17357799 maglogo: N pubinfo: dt: Apr2020 vid: 14 iid: 2 pid: 50887 pub: Qom University of Medical Sciences artinfo: ui: 144695606 144695606 144695606 10.29252/qums.14.2.1 144695606 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: anti-CD25 CAR مرتبط با qRT-PCR و RNA تجزیه وتحلیل های ساختار ثانویه NK- بیان شده در رده ی سلولی 92 aug: au: معین دهباشی زهره حجتی مجید متولی باشی مزدک گنجعلی خانی حاکمی affil: بخش ژنتیک، گروه زیست شناسی سلولی و مولکولی و میکروبیولوژی، دانشکده علوم و فناوری های زیستی، دانشگاه اصفهان، اصفهان، ایران sug: subj: RNA Analysis Receptors, Cell Surface Physiology Killer Cells, Natural Cell Line, Tumor Biological Markers Human Receptors, Immunologic Polymerase Chain Reaction Methods Bioinformatics ab: Background and Objectives: Tumor-infiltrating regulatory T (TI-Treg) cells perform an important function in cancer immune escape. In this study, the third generation CAR construct was designed against human CD25 antigen as the cell surface biomarker of TI-Treg cells. Methods: At first, the construct of anti-CD25 CAR was designed. Using RNAfold web server, the RNA secondary structure was evaluated. Also, NK-92 cell line was transduced using lentiviral vectors. Then, the expression level of anti-CD25 CAR RNA, was assessed by qRT-PCR in NK-92 cells transduced with CAR and mock transfer vectors as well as untreated cells. Results: The RNA secondary structure was stable. Also, the expression level of anti-CD25 CAR RNA in the NK-92 cells transduced by pCDH-513B-1-anti-CD25 CAR transfer vector was significantly higher than NK-92 cells transduced by mock transfer vector and untreated cells (P˂0.0001). Conclusion: The present study on anti-CD25 CAR RNA showed that this type of CAR transcripts were stable and expressed at a high level. In fact, this type of CAR can be further studied in the future as a tool to remove the cancer immune escape in all types of solid and liquid cancers. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Persian refInfo: holdings: @attributes: islocal: N |
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