Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry.
Objective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric...
| Publicado en: | BioMed Research International pp. 1 - 9 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Wiley-Blackwell
8/5/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=144948050&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 144948050 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 8/5/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 144948050 144948050 144948050 10.1155/2020/2743060 144948050 ppf: 1 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Study on the Diagnosis of Gastric Cancer by Magnetic Beads Extraction and Mass Spectrometry. aug: au: Zhu, Ning Xing, Xiaoliang Cao, Limei Zhang, Yingjun Zhang, Ti Li, Zhen Zou, Fen Li, Qing affil: Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Public Health and Laboratory Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, China sug: subj: Mass Spectrometry Methods Early Detection of Cancer Methods Peptides Blood Neoplasm Staging Stomach Neoplasms Diagnosis Human Randomized Controlled Trials Comparative Studies Neoplasm Recurrence, Local Diagnosis Age Factors Sex Factors Reliability Antigens, Tumor Blood Carbohydrates Blood Gene Expression Evaluation Sensitivity and Specificity ab: Objective. This study constructed a model for the early diagnosis of gastric cancer by comparing the serum peptides profiles of patients with advanced gastric cancer and healthy people. And that model may be the potential to be applied for the efficacy evaluation and recurrence monitoring in gastric cancer. Methods. Serums of 30 healthy people and 30 advanced gastric cancer patients were matched by age and gender were collected. The serum peptide spectrum was obtained by MB-WCX concentration and MALDI-TOF MS analysis. Based on the analysis of the efficiency of differential peptides in the diagnosis of gastric cancer, we first established a model for the diagnosis of gastric cancer based on differential peptides and then carried out external verification. The diagnostic reliability of this model was further tested by compared with carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). Results. In this present study, we found the expression of two peptide peaks with a molecular weight of 2863 Da and 2953 Da were significantly increased in gastric cancer serum, while the expression of two peptide peaks with a molecular weight of 1945 Da and 2082 Da were significantly decreased. Depending on the characteristics of peptide expression, we constructed a diagnostic model, we compared the sensitivity and specificity of the model established by 2953 Da/1945 Da, and found this model is significantly higher than CEA and CA19-9. Conclusion. There were some differences in serum peptides profiles between patients with advanced gastric cancer and healthy people. The serum peptide diagnostic models based on 2953 Da and 1945 Da have high diagnostic efficiency for advanced gastric cancer. Our result indicated that this model was well worth further validation for clinical diagnosis. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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