An in vitro model of temporal enhancement of epithelium barrier permeability by low-energy shock waves without contrast agents.
One of the commonly used techniques for drug delivery is to temporarily increase the permeability of tissue barriers. Acoustic energies such as ultrasound and shock waves are known to modulate tissue permeability. Recently, it was found that shock waves modulate the blood-brain barrier in the rat br...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 58; no. 9; pp. 1987 - 1994 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145048077&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145048077 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Sep2020 vid: 58 iid: 9 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 145048077 144441308 145048077 NLM32591989 10.1007/s11517-020-02207-8 NLM32591989 145048077 ppf: 1987 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: An in vitro model of temporal enhancement of epithelium barrier permeability by low-energy shock waves without contrast agents. aug: au: Monden, Yuki Tsukamoto, Akira Ushida, Takashi Kobayashi, Etsuko Nakagawa, Keiichi Sakuma, Ichiro affil: Department of Precision Engineering, Graduate School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan sug: subj: Drug Delivery Systems Epithelium Metabolism Sound Permeability In Vitro Studies Rats Dogs Electric Impedance Animals Biomedical Engineering Cell Line ab: One of the commonly used techniques for drug delivery is to temporarily increase the permeability of tissue barriers. Acoustic energies such as ultrasound and shock waves are known to modulate tissue permeability. Recently, it was found that shock waves modulate the blood-brain barrier in the rat brain without injection of contrast agents such as microbubbles. This finding implies that modulation of other tissue barriers by shock wave exposure without contrast agents may be possible. To examine whether the modulation is also possible with other tissue barriers, we here investigated whether shock waves would modulate an in vitro tissue barrier model consisting of epithelial cells cultured on culture inserts. The permeability of the epithelium sheets evaluated by trans-epithelial electrical resistance (TEER) was increased following shock waves at a peak pressure of 11 MPa. The increased permeability recovered within 2 h. This enhancement was realized with one-shot low-energy shock waves having an acoustic energy of 0.013 mJ/mm2. Monitoring the peak pressures in every exposure revealed that the minimum peak pressure required for the enhancement is 2.9 MPa. These results indicate that shock wave exposure has the potential to temporarily increase the permeability of epithelium barriers to enhance drug delivery without contrast agents. Graphical abstract Enhancements of epithelial barrier permeability were evaluated by trans-epithelial electrical resistance (TEER) before and after shock wave exposures. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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