Evaluation of cell blocks from effusion specimens in Gynecologic Oncopathology: An experience of 220 cases, diagnosed at a Tertiary Cancer Referral Center.
One of the common indications of ascitic fluid examination in gynecological oncopathology is the detection and classification of malignant cells, especially in cases of clinically suspicious tubo-ovarian masses. The present study was undertaken to assess and validate the diagnostic utility of cell b...
| Published in: | Indian Journal of Pathology & Microbiology Vol. 63; no. 3; pp. 427 - 435 |
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| Main Authors: | , , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wolters Kluwer India Pvt Ltd
Jul-Sep2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145106790&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145106790 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03774929 50DI jtl: Indian Journal of Pathology & Microbiology issn: 03774929 maglogo: N pubinfo: dt: Jul-Sep2020 vid: 63 iid: 3 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 145106790 145106790 NLM32769333 145106790 10.4103/IJPM.IJPM_858_19 NLM32769333 145106790 ppf: 427 ppct: 8 formats: tig: atl: Evaluation of cell blocks from effusion specimens in Gynecologic Oncopathology: An experience of 220 cases, diagnosed at a Tertiary Cancer Referral Center. aug: au: Rekhi, Bharat Karmarkar, Srushti Gupta, Chhavi Deodhar, Kedar Menon, Santosh Pathuthara, Saleem Maheshwari, Amita Shylasree, T Gupta, Sudeep Deodhar, Kedar K Shylasree, T S affil: Department of Surgical Pathology, Division of Cytopathology, Tata Memorial Center, HBNI University, Mumbai, Maharashtra sug: subj: Ovarian Neoplasms Diagnosis Adenocarcinoma Diagnosis Mesothelioma Pathology Pleural Effusion Pathology Body Fluids Mesothelioma Diagnosis Ovarian Neoplasms Pathology Retrospective Design Middle Age Female Prospective Studies Adenocarcinoma Immunohistochemistry Referral and Consultation Hospitals, Special Human Middle Aged: 45-64 years Female ab: One of the common indications of ascitic fluid examination in gynecological oncopathology is the detection and classification of malignant cells, especially in cases of clinically suspicious tubo-ovarian masses. The present study was undertaken to assess and validate the diagnostic utility of cell blocks (CBs) and compare its results with the corresponding conventional smears, prepared from effusion samples. CBs were prepared by thromboplastin technique in 220 cases. In 208 cases, diagnostic concordance between results obtained from smears and corresponding CBs was evaluated. Various antibody markers were tested, as per individual case. The average age of patients was 52.2 years. Positive immunohistochemical (IHC) staining for various markers was observed in 182 cases (82.7%) The most frequently positive antibody marker was PAX8 (101/134), followed by p53 (85/92) [mutation type (either diffusely positive or completely negative)], WT1 (tumor cells) (80/112), calretinin (2/87) (diffuse), BerEP4 (21/49), CA125 (21/24), CK7 (31/39) and CK20 and CDX2, together (5/16). Various other IHC markers utilized, including their positive expression, were TTF1 (1/10), p40 (3/3), p63 (2/4), ER (21/29), HBME1 (1/7), GATA3 (1/4), and MIC2 (1/1). Complete diagnostic concordance between CBs and smears was observed in 170/208 cases (81.7%). There were 20 major discordances, 10 minor and 8 cases with sampling errors. IHC was useful in classifying 158/182 (86.8%) cases, including serous or Müllerian adenocarcinoma (n = 123), mostly high-grade (121); metastatic squamous carcinoma (3); gastrointestinal-type adenocarcinoma (8); pulmonary adenocarcinoma (1); breast adenocarcinoma (1); Ewing sarcoma (1); and mesothelioma (2). CBs are complementary to smears in the detection of gynecological malignancies, mostly high-grade serous adenocarcinomas. These provide an opportunity for testing several IHC markers, for a precise diagnosis, including in various uncommon case scenarios, associated with significant therapeutic implications. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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