HER2 expression on tumor-derived extracellular vesicles and circulating tumor cells in metastatic breast cancer.
Background: Tumor-derived extracellular vesicles (tdEVs) and circulating tumor cells (CTCs) in the blood of metastatic cancer patients associate with poor outcomes. In this study, we explored the human epidermal growth factor receptor 2 (HER2) expression on CTCs and tdEVs of metastatic breast cancer...
| Publicado en: | Breast Cancer Research Vol. 22; no. 1; pp. 1 - 12 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
8/12/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145108296&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145108296 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14655411 8UYJ jtl: Breast Cancer Research issn: 14655411 maglogo: N pubinfo: dt: 8/12/2020 vid: 22 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 145108296 145108296 NLM32787900 145108296 10.1186/s13058-020-01323-5 NLM32787900 145108296 ppf: 1 ppct: 11 formats: fmt: @attributes: type: P tig: atl: HER2 expression on tumor-derived extracellular vesicles and circulating tumor cells in metastatic breast cancer. aug: au: Nanou, Afroditi Zeune, Leonie Laura Bidard, Francois-Clement Pierga, Jean-Yves Terstappen, Leonardus Wendelinus Mathias Marie affil: Department of Medical Cell BioPhysics, Faculty of Science and Technology, University of Twente, Carré Room CR4433, Hallenweg 23, 7522 NH, Enschede, The Netherlands sug: subj: Neoplasm Metastasis Metabolism Receptors, Cell Surface Metabolism Breast Neoplasms Pathology Female Neoplasm Metastasis Breast Neoplasms Metabolism Human Prognosis Breast Neoplasms Proteins Metabolism ROC Curve Survival Validation Studies Comparative Studies Evaluation Research Multicenter Studies Scales Female ab: Background: Tumor-derived extracellular vesicles (tdEVs) and circulating tumor cells (CTCs) in the blood of metastatic cancer patients associate with poor outcomes. In this study, we explored the human epidermal growth factor receptor 2 (HER2) expression on CTCs and tdEVs of metastatic breast cancer patients.Methods: Blood samples from 98 patients (CLCC-IC-2006-04 study) were originally processed with the CellSearch® system using the CTC kit and anti-HER2 as an additional marker in the staining cocktail. CTCs and tdEVs were automatically enumerated from the generated CellSearch images using the open-source ACCEPT software.Results: CTCs and tdEVs were subdivided based on their cytokeratin (CK) and HER2 phenotype into CK+HER2-, CK-HER2+, and CK+HER2+. The inclusion of anti-HER2 increased the percentage of informative samples with ≥ 1 detectable CTC from 89 to 95%. CK- CTCs and tdEVs correlated equally well with the clinical outcome as CK+ CTCs and tdEVs. Inter- and intra-patient heterogeneity was found for the CTC/tdEV phenotypes, and the presence of 2 or 3 classes of CTCs/tdEVs was associated with worse prognosis compared to a uniform CTC/tdEV phenotype present (1 class). The use of ≥ 7% HER2+CK+ tdEVs can predict HER2 expression of the tissue with 74% sensitivity and specificity using the HER2 amplification status of the primary tumor as a classification variable.Conclusions: HER2 can be detected on CTCs and tdEVs not expressing CK, and these CK- CTCs/tdEVs have similar clinical relevance to CTCs and tdEVs expressing CK. tdEVs perform better than CTCs in predicting the HER2 status of the primary tissue. CTC and tdEV heterogeneity in the blood of patients is inversely associated with overall survival. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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