Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis.
Background: Temporomandibular joint osteoarthritis (TMJOA) is a progressive degenerative disease caused by imbalance between anabolic and catabolic stimuli. Objective: The aim of this study was to evaluate histopathological changes, collagen degeneration and the expression of eleven TMJOA biomarkers...
| Publicado en: | Journal of Oral Rehabilitation Vol. 47; no. 9; pp. 1084 - 1095 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145255654&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145255654 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0305182X ERZ jtl: Journal of Oral Rehabilitation issn: 0305182X maglogo: Y pubinfo: dt: Sep2020 vid: 47 iid: 9 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 145255654 145255654 145830914 145255654 10.1111/joor.13034 145255654 ppf: 1084 ppct: 11 formats: tig: atl: Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis. aug: au: Fernandes, Bruno V. Brancher, João A. Michels, Arieli C. Nagashima, Seigo Johann, Aline Cristina B. R. Bóia Ferreira, Marianna Costa, Delson J. Rebellato, Nelson Luis B. Klüppel, Leandro E. Scariot, Rafaela Zielak, João C. affil: School of Health Sciences, Universidade Positivo, Curitiba, Brazil sug: subj: Temporomandibular Joint Diseases Osteoarthritis Immunohistochemistry Biological Markers Physiology Temporomandibular Joint Anatomy and Histology Collagen Diseases Human Neovascularization, Physiologic Female Spearman's Rank Correlation Coefficient Descriptive Statistics Biological Markers Blood Female ab: Background: Temporomandibular joint osteoarthritis (TMJOA) is a progressive degenerative disease caused by imbalance between anabolic and catabolic stimuli. Objective: The aim of this study was to evaluate histopathological changes, collagen degeneration and the expression of eleven TMJOA biomarkers in articular discs. Methods: Specimens were obtained from eight female patients submitted to discectomy. Discs were divided into anterior band (AB), intermediate zone (IZ) and posterior band (PB) for computerised histomorphometric analyses. Each was assigned a histopathological degeneration score (HDS). Collagen degeneration was assessed with Picrosirius‐polarisation method. Biomarkers were evaluated through immunohistochemistry, including IGF‐1, OPG, VEGF, TNF‐α, FGF‐23, IHH, MMP‐3, MMP‐9, TGF‐β1, BMP‐2 and WNT‐3. Image processing software was used to calculate average immature collagen ratios and immunostained areas. Spearman rank tests were applied to verify correlations, with significance level of 0.05. Results: The HDS showed negative correlation with expression of VEGF in IZ and PB (P <.05) and positive with TNF‐α in AB (P <.01). Collagen degeneration correlated with TGF‐β1 (P <.05), BMP‐2 (P <.01) and IHH (P <.05) immunostained areas in the IZ; TGF‐β1, BMP‐2 and IHH expression correlated among each other in AB and IZ (P <.05). Conclusion: Angiogenesis and tissue fragmentation may result from aberrant physiologic responses mediated by VEGF and TNF‐α, compromising TMJ discs during OA progression. The expression of TGF‐β1, BMP‐2 and IHH could be related to collagen degeneration in displaced discs and may participate in TMJOA pathogenesis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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