A Modified Murine Calvarial Osteolysis Model Exposed to Ti Particles in Aseptic Loosening.
Aim. To investigate the different effects on osteolysis between commercial pure Ti particles and TiAl6V4 particles obtained from prosthesis of patients with aseptic loosening. Method. Scanning electron microscope, energy dispersive X-ray spectrometry, and X-ray diffraction were used for the size tes...
| Publicado en: | BioMed Research International pp. 1 - 8 |
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| Autores principales: | , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
8/25/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145298106&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145298106 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 8/25/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 145298106 145298106 145298106 10.1155/2020/3403489 145298106 ppf: 1 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A Modified Murine Calvarial Osteolysis Model Exposed to Ti Particles in Aseptic Loosening. aug: au: Deng, Zhantao Wang, Shuai Li, Mengyuan Fu, Guangtao Liu, Chang Li, Shuxian Jin, Jiewen Lyu, Feng-Juan Ma, Yuanchen Zheng, Qiujian affil: Department of Orthopedics, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China sug: subj: Titanium Analysis Joint Prosthesis Prosthesis Failure Osteolysis Models, Biological Animal Studies Rats Microscopy, Electron, Scanning Crystallography Spectrometry, X-Ray Emission Tomography, X-Ray Computed Imaging, Three-Dimensional Staining and Labeling ab: Aim. To investigate the different effects on osteolysis between commercial pure Ti particles and TiAl6V4 particles obtained from prosthesis of patients with aseptic loosening. Method. Scanning electron microscope, energy dispersive X-ray spectrometry, and X-ray diffraction were used for the size test, chemical composition test, and phase analysis of two kinds of Ti particles. Microcomputed tomography (micro-CT) and 3-dimensional reconstruction analysis were applied to analyze the bone loss quantitatively and radiologically. Hematoxylin-eosin (HE) staining and tartrate-resistant acid phosphatase (TRAP) staining were used to assess the histologic difference. Result. TiAl6V4 particles were constituted by FeO, Al45V7, and Al3Ti while pure Ti particles were constituted by Ti, Ti3O, and C4H7NO3. Similar particle size of nanoscale was detected of two Ti particles. A TiAl6V4 osteolysis model had more severe bone loss when scanned with micro-CT and assessed by quantitative analysis. TiAl6V4 also presented deeper and wider calvarial bone loss in HE staining and more activated osteoclasts in TRAP staining. Conclusion. A mouse calvarial model is the most effective animal model for the primary in vivo research of aseptic loosening. Compared with commercial Ti particles, TiAl6V4 particles derived from prosthesis of an aseptic loosening patient had more severe bone loss and more activated osteoclast, which was more consistent with pathogenesis of aseptic loosening in vivo, had high success rate of establishment of a model, and was more desired in animal modeling. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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