Ingestion of gastrolith mineralized matrix increases bone volume and tissue volume in mouse long bone fracture model.
Fracture healing often requires extended convalescence as the bony fragments consolidate into restored viable tissue for load-bearing. Development of interventions to improve healing remains a priority for orthopaedic research. The goal of this study was to evaluate the ability of a naturally occurr...
| Publicado en: | Journal of Orthopaedics Vol. 20; pp. 251 - 257 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Professor PK Surendran Memorial Education Foundation
Jul2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145406321&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145406321 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 25899082 1CUL jtl: Journal of Orthopaedics issn: 25899082 maglogo: N pubinfo: dt: Jul2020 vid: 20 pid: 24594 pub: Professor PK Surendran Memorial Education Foundation artinfo: ui: 145406321 145406321 145406321 10.1016/j.jor.2020.01.036 145406321 ppf: 251 ppct: 6 formats: fmt: @attributes: type: T tig: atl: Ingestion of gastrolith mineralized matrix increases bone volume and tissue volume in mouse long bone fracture model. aug: au: Wenger, Karl H. Zumbrun, Steven D. Rosas, Militza Dickinson, Douglas P. McPherson III, James C. affil: Department of Clinical Investigation, Dwight D. Eisenhower Army Medical Center, Fort Gordon, 30905, Georgia sug: subj: Calcium Carbonate Administration and Dosage Dietary Supplementation Bone Density Connective Tissue Fibula Fractures Therapy Fracture Healing Treatment Outcomes Animal Studies Mice Descriptive Statistics Comparative Studies Osteogenesis ab: Fracture healing often requires extended convalescence as the bony fragments consolidate into restored viable tissue for load-bearing. Development of interventions to improve healing remains a priority for orthopaedic research. The goal of this study was to evaluate the ability of a naturally occurring matrix of amorphous calcium carbonate to affect fracture healing in an uninstrumented long bone model. Complete transverse fracture was induced in the fibula of mature mice, followed by daily gavage of crushed gastrolith from crayfish at doses of 0 (control), 1 (1 MG), and 5 (5 MG) mg/kg. At Day 17, bones and sera were harvested. Morphologically, the 1 MG treated group had greater bone volume (BV), and both 1 MG and 5 MG had greater tissue volume (TV) than control (p < 0.05), as determined by μCT; BV/TV and mineral density did not yield a statistical difference. Histologically, regional variations in mineralized matrix were evident in all specimens, indicating a broad continuum of healing within the callus. Among serum proteins, bone-specific alkaline phosphatase, indicative of active mineralization, was greater in 5 MG than control (p < 0.05). Sclerostin, an inhibitor of osteogenesis, was lower in 5 MG than control (p < 0.05), also suggestive of enhanced healing. An increase in bone volume, tissue volume and cellular signaling for osteogenesis at 17 days following fibula fracture in this mouse model suggests that gastrolith treatment holds potential for improving fracture healing. Further study at subsequent time points is warranted to determine the extent to which the increase in callus size with gastrolith treatment may accelerate restoration of tissue integrity. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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