Microbiome Diagnostics.
During the past decade, breakthroughs in sequencing technology and computational biology have provided the basis for studies of the myriad ways in which microbial communities (“microbiota”) in and on the human body influence human health and disease. In almost every medical specialty, there is now a...
| Publicado en: | Clinical Chemistry Vol. 66; no. 1; pp. 68 - 77 |
|---|---|
| Autor principal: | |
| Formato: | Journal Article |
| Publicado: |
Oxford University Press / USA
Jan2020
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145704303&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145704303 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00099147 10CS jtl: Clinical Chemistry issn: 00099147 maglogo: N pubinfo: dt: Jan2020 vid: 66 iid: 1 pid: 622 pub: Oxford University Press / USA artinfo: ui: 145704303 10.1373/clinchem.2019.303248 145704303 ppf: 68 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Microbiome Diagnostics. aug: au: Schlaberg, Robert affil: Department of Pathology, University of Utah, Salt Lake City, UT. sug: ab: During the past decade, breakthroughs in sequencing technology and computational biology have provided the basis for studies of the myriad ways in which microbial communities (“microbiota”) in and on the human body influence human health and disease. In almost every medical specialty, there is now a growing interest in accurate and replicable profiling of the microbiota for use in diagnostic and therapeutic application. CONTENT: This review provides an overview of approaches, challenges, and considerations for diagnostic applications borrowing from other areas of molecular diagnostics, including clinical metagenomics. Methodological considerations and evolving approaches for microbiota profiling from mitochondrially encoded 16S rRNA-based amplicon sequencing to metagenomics and metatranscriptomics are discussed. To improve replicability, at least the most vulnerable steps in testing workflows will need to be standardized and continuous efforts needed to define QC standards. Challenges such as purity of reagents and consumables, improvement of reference databases, and availability of diagnostic-grade data analysis solutions will require joint efforts across disciplines and with manufacturers. SUMMARY: The body of literature supporting important links between the microbiota at different anatomic sites with human health and disease is expanding rapidly and therapeutic manipulation of the intestinal microbiota is becoming routine. The next decade will likely see implementation of microbiome diagnostics in diagnostic laboratories to fully capitalize on technological and scientific advances and apply them in routine medical practice pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|