Digital PCR—An Emerging Technology with Broad Applications in Microbiology.
The PCR and its variant, quantitative PCR (qPCR), have revolutionized the practice of clinical microbiology. Continued advancements in PCR have led to a new derivative, digital PCR (dPCR), which promises to address certain limitations inherent to qPCR. CONTENT: Here we highlight the important techni...
| Publicado en: | Clinical Chemistry Vol. 66; no. 1; pp. 117 - 124 |
|---|---|
| Autores principales: | , |
| Formato: | Journal Article |
| Publicado: |
Oxford University Press / USA
Jan2020
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145704308&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145704308 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00099147 10CS jtl: Clinical Chemistry issn: 00099147 maglogo: N pubinfo: dt: Jan2020 vid: 66 iid: 1 pid: 622 pub: Oxford University Press / USA artinfo: ui: 145704308 10.1373/clinchem.2019.304048 145704308 ppf: 117 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Digital PCR—An Emerging Technology with Broad Applications in Microbiology. aug: au: Salipante, Stephen J. Jerome, Keith R. affil: Department of Laboratory Medicine, University of Washington, Seattle, WA. sug: ab: The PCR and its variant, quantitative PCR (qPCR), have revolutionized the practice of clinical microbiology. Continued advancements in PCR have led to a new derivative, digital PCR (dPCR), which promises to address certain limitations inherent to qPCR. CONTENT: Here we highlight the important technical differences between qPCR and dPCR, and the potential advantages and disadvantages of each. We then review specific situations in which dPCR has been implemented in clinical microbiology and the results of such applications. Finally, we attempt to place dPCR in the context of other emerging technologies relevant to the clinical laboratory, including next-generation sequencing. SUMMARY: dPCR offers certain clear advantages over traditional qPCR, but these are to some degree offset by limitations of the technology, at least as currently practiced. Laboratories considering implementation of dPCR should carefully weigh the potential advantages and disadvantages of this powerful technique for each specific application planned. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|