Volumetric Optoacoustic Tomography Differentiates Myocardial Remodelling.
Purpose: Myocardial healing following myocardial infarction (MI) is a complex process that is yet to be fully understood. Clinical attempts in regeneration of the injured myocardium using cardiac stem cells faced major challenges, calling for a better understanding of the processes involved at a mor...
| Publicado en: | Molecular Imaging & Biology Vol. 22; no. 5; pp. 1235 - 1244 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Oct2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=145733571&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 145733571 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2020 vid: 22 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 145733571 144056200 145733571 NLM32394284 10.1007/s11307-020-01498-5 NLM32394284 145733571 ppf: 1235 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Volumetric Optoacoustic Tomography Differentiates Myocardial Remodelling. aug: au: Ivankovic, Ivana Déan-Ben, Xosé Luís Haas, Helena Kimm, Melanie A. Wildgruber, Moritz Razansky, Daniel affil: Faculty of Medicine and Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland sug: subj: Acoustics Myocardium Pathology Tomography Heart Heart Physiopathology Arrhythmia Physiopathology Myocardial Infarction Complications Arrhythmia Myocardial Infarction Mice Myocardial Reperfusion Indoles Models, Biological Animals Arrhythmia Complications Myocardial Infarction Physiopathology Clinical Assessment Tools Scales ab: Purpose: Myocardial healing following myocardial infarction (MI) is a complex process that is yet to be fully understood. Clinical attempts in regeneration of the injured myocardium using cardiac stem cells faced major challenges, calling for a better understanding of the processes involved at a more basic level in order to foster translation.Procedures: We examined the feasibility of volumetric optoacoustic tomography (VOT) in studying healing of the myocardium in different models of MI, including permanent occlusion (PO) of the left coronary artery, temporary occlusion (ischemia-reperfusion-I/R) and infarcted c-kit mutants, a genetic mouse model with impaired cardiac healing. Murine hearts were imaged at 100 Hz frame rate using 800 nm excitation wavelength, corresponding to the peak absorption of indocyanine green (ICG) in plasma and the isosbestic point of haemoglobin.Results: The non-invasive real-time volumetric imaging capabilities of VOT have allowed the detection of significant variations in the pulmonary transit time (PTT), a parameter affected by MI, across different murine models. Upon intravenous injection of ICG, we were able to track alterations in cardiac perfusion in I/R models, which were absent in wild-type (wt) PO or kitW/kitW-v PO mice. The wt-PO and I/R models further exhibited irregularities in their cardiac cycles.Conclusions: Clear differences in the PTT, ICG perfusion and cardiac cycle patterns were identified between the different models and days post MI. Overall, the results highlight the unique capacity of VOT for multi-parametric characterization of morphological and functional changes in murine models of MI. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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