The Anatase Phase of Nanotopography Titania with Higher Roughness Has Better Biocompatibility in Osteoblast Cell Morphology and Proliferation.

Previous studies have concluded that surface-modified titanium oxide (titania, TiO2) surface properties promote osteoblast cell morphology and proliferation. To screen a suitable structured titania coating with the best biocompatibility to be used in dental implants, five titania films (two amorphou...

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Publicado en:BioMed Research International pp. 1 - 9
Autores principales: Ruan, Danping, Wu, Chunyun, Deng, Sinan, Zhang, Yu, Guan, Guoling
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 9/22/2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 9/22/2020
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2020/8032718
        146010998
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        atl: The Anatase Phase of Nanotopography Titania with Higher Roughness Has Better Biocompatibility in Osteoblast Cell Morphology and Proliferation.
      aug:
        au:
          Ruan, Danping
          Wu, Chunyun
          Deng, Sinan
          Zhang, Yu
          Guan, Guoling
        affil: Minhang Branch, Zhongshan Hospital, Fudan University, China
      sug:
        subj:
          Titanium
          Biocompatible Materials
          Osteoblasts
          Cell Proliferation
          Dental Implants
          Radio Frequency Identification
          Microscopy
          X-Rays
          Mice
          Animal Studies
          Materials Testing
      ab: Previous studies have concluded that surface-modified titanium oxide (titania, TiO2) surface properties promote osteoblast cell morphology and proliferation. To screen a suitable structured titania coating with the best biocompatibility to be used in dental implants, five titania films (two amorphous, one rutile, and two anatases) with different surfaces were successfully synthesized on polished titanium by radio frequency (RF) magnetron sputtering. We applied atomic force microscopy (AFM) and X-ray diffraction (XRD) to depict the formulations. Furthermore, MC3T3-E1, the mouse osteoblast precursor cell, was used to assess cell proliferation and observe morphologic changes at the film surface. The data indicated that the overall number of MC3T3-E1 cells on anatase films was significantly higher as compared with cells on rutile and amorphous films. Meanwhile, the actin filaments of the cells grown on the anatase phase films were well defined and fully spread. In addition, the film with higher roughness had enhanced biocompatibility than that with lower roughness. The results showed that the crystal phase and titania coated roughness had a greater influence on the biocompatibility of nanostructured titania film. The higher the roughness of the anatase phase was, the better bioactivity for the morphology and proliferation of osteoblast. This is a good surface-modified biological material and may have a good application prospect in dental implants.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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