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Molecular identification of Cryptococcus gattii from cerebrospinal fluid using single-cell sequencing: A case study.

A 31-year-old man presented with cryptococcal meningitis (CM) without typical clinical characteristics, but with abnormal walking, difficult leg lifting and frequent falling. He was admitted to Peking Union Medical College Hospital. After multiple tests failed to identify the pathogen, single-cell s...

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Published in:Journal of Infection Vol. 81; no. 4; pp. 634 - 639
Main Authors: Chen, Min, Hong, Nan, Hu, Shan, Wang, Peng, Guan, HongZhi, Xiao, Meng, Zhu, Xinlin, Al-Hatmi, Abdullah M.S., Zhou, Zhe, Gao, Lei, Boekhout, Teun, Xu, Jianping, Xu, Yingchun, Liao, Wanqing, Yang, Ying
Format: research Journal Article
Published: W B Saunders Oct2020
Online Access:View this record in EBSCOhost
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      dt: Oct2020
      vid: 81
      iid: 4
      pid: 1351
      pub: W B Saunders
      place: Philadelphia, Pennsylvania
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        146013304
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        NLM32590007
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        10.1016/j.jinf.2020.06.040
        NLM32590007
        146013304
      ppf: 634
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        atl: Molecular identification of Cryptococcus gattii from cerebrospinal fluid using single-cell sequencing: A case study.
      aug:
        au:
          Chen, Min
          Hong, Nan
          Hu, Shan
          Wang, Peng
          Guan, HongZhi
          Xiao, Meng
          Zhu, Xinlin
          Al-Hatmi, Abdullah M.S.
          Zhou, Zhe
          Gao, Lei
          Boekhout, Teun
          Xu, Jianping
          Xu, Yingchun
          Liao, Wanqing
          Yang, Ying
        affil: Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China
      sug:
        subj:
          Cryptococcosis Diagnosis
          Meningitis, Cryptococcal Diagnosis
          Cryptococcus
          Human
          Male
          Genotype
          Adult
          Comparative Studies
          Multicenter Studies
          Evaluation Research
          Validation Studies
          Scales
          Adult: 19-44 years
          Male
      ab: A 31-year-old man presented with cryptococcal meningitis (CM) without typical clinical characteristics, but with abnormal walking, difficult leg lifting and frequent falling. He was admitted to Peking Union Medical College Hospital. After multiple tests failed to identify the pathogen, single-cell sequencing (scS) was used to test the cerebrospinal fluid (CSF). Comparing the sequence obtained from single-cell sequencing with the reference database, it was found that the infection was caused by Cryptococcus gattii sensu stricto (AFLP4/VGI genotype). Cryptococcus is difficult to cultivate from complex body fluids. The etiological agent of this patient was identified and the patient was treated. This is the first case in which scS was used to detect and identify fungal pathogen after conventional testing failed to identify the cause of the disease. This report demonstrates that the scS approach can be used to generate fungal genome sequences directly from the CSF of a CM patient. The scS technology could become a powerful tool to precise detect microscopically visible but uncultured pathogens in clinical samples.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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