Determining factors for optimal neuronal and glial Golgi-Cox staining.
Golgi staining allows for the analysis of neuronal arborisations and connections and is considered a powerful tool in basic and clinical neuroscience. The fundamental rules for improving neuronal staining using the Golgi-Cox method are not fully understood; both intrinsic and extrinsic factors may c...
| Published in: | Histochemistry & Cell Biology Vol. 154; no. 4; pp. 431 - 449 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Published: |
Springer Nature
Oct2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=146224944&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 146224944 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Oct2020 vid: 154 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 146224944 143954974 10.1007/s00418-020-01891-9 146224944 ppf: 431 ppct: 18 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Determining factors for optimal neuronal and glial Golgi-Cox staining. aug: au: Narayanan, Sareesh Naduvil Bairy, Laxminarayana Kurady Srinivasamurthy, Suresh Kumar affil: Department of Physiology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, PO Box 11172, Ras Al Khaimah, United Arab Emirates sug: ab: Golgi staining allows for the analysis of neuronal arborisations and connections and is considered a powerful tool in basic and clinical neuroscience. The fundamental rules for improving neuronal staining using the Golgi-Cox method are not fully understood; both intrinsic and extrinsic factors may control the staining process. Therefore, various conditions were tested to improve the Golgi-Cox protocol for vibratome-cut rat brain sections. Optimal staining of cortical neurons was achieved after 72 h of impregnation. Well-stained neurons in both cortical and subcortical structures were observed after 96 h of impregnation. The dendritic arborisation pattern of cortical neurons derived from the 72-h impregnation group was comparable to those of the 96 and 168-h impregnation groups. The entire brain was stained well when the pH of the Golgi-Cox solution was 6.5 and that of the sodium carbonate solution was 11.2. Lack of brain perfusion or perfusion with 0.9% NaCl did not influence optimal neuronal staining. Perfusion with 37% formaldehyde, followed by impregnation, only resulted in glial staining, but perfusion with 4% formaldehyde facilitated both glial and neuronal staining. Whole brains required longer impregnation times for better staining. Although every factor had a role in determining optimal neuronal staining, impregnation time and the pH of staining solutions were key factors among them. This modified Golgi-Cox protocol provides a simple and economical procedure to stain both neurons and glia separately. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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