Protection of Pancreatic Islets Using Theranostic Silencing Nanoparticles in a Baboon Model of Islet Transplantation.
The long-term success of pancreatic islet transplantation (Tx) as a cure for type 1 diabetes remains limited. Islet loss after Tx related to apoptosis, inflammation, and other factors continues to limit Tx efficacy. In this project, we demonstrate a novel approach aimed at protecting islets before T...
| Publicado en: | Diabetes Vol. 69; no. 11; pp. 2414 - 2423 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
American Diabetes Association
Nov2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=146510567&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 146510567 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00121797 1G6 jtl: Diabetes issn: 00121797 maglogo: N pubinfo: dt: Nov2020 vid: 69 iid: 11 pid: 1367 pub: American Diabetes Association place: Arlington, Virginia artinfo: ui: 146510567 146510567 NLM32855170 146510567 10.2337/db20-0517 NLM32855170 146510567 ppf: 2414 ppct: 9 formats: tig: atl: Protection of Pancreatic Islets Using Theranostic Silencing Nanoparticles in a Baboon Model of Islet Transplantation. aug: au: Pomposelli, Thomas Wang, Ping Takeuchi, Kazuhiro Miyake, Katsunori Ariyoshi, Yuichi Watanabe, Hironosuke Chen, Xiaojuan Shimizu, Akira Robertson, Neil Yamada, Kazuhiko Moore, Anna Xiaojuan, Chen affil: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY sug: subj: Genes Nanoparticles Islets of Langerhans Transplantation Primates Animal Studies Islets of Langerhans Metabolism Funding Source ab: The long-term success of pancreatic islet transplantation (Tx) as a cure for type 1 diabetes remains limited. Islet loss after Tx related to apoptosis, inflammation, and other factors continues to limit Tx efficacy. In this project, we demonstrate a novel approach aimed at protecting islets before Tx in nonhuman primates (NHPs) (baboons) by silencing a gene (caspase-3) responsible for induction of apoptosis. This was done using siRNA (siCas-3) conjugated to magnetic nanoparticles (MNs). In addition to serving as carriers for siCas-3, these nanoparticles also act as reporters for MRI, so islets labeled with MN-siCas-3 can be monitored in vivo after Tx. In vitro studies showed the antiapoptotic effect of MN-siCas-3 on islets in culture, resulting in minimal islet loss. For in vivo studies, donor baboon islets were labeled with MN-siCas-3 and infused into recipient diabetic subjects. A dramatic reduction in insulin requirements was observed in animals transplanted with even a marginal number of labeled islets compared with controls. By demonstrating the protective effect of MN-siCas-3 in the challenging NHP model, this study proposes a novel strategy to minimize the number of donor islets required from either cadaveric or living donors. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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