Preparation and Standardization of Escherichia coli Nosodes Sourced from Select E. coli Strains.
Background The nosodes are well-known preparations in homeopathy that are sourced from organisms and diseased materials. More than 40 known nosodes have been used in homeopathic practice for over a century. Having identified the need for scientifically developed new nosodes sourced from organisms t...
| Publicado en: | Homeopathy Vol. 109; no. 4; pp. 207 - 213 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Thieme Medical Publishing Inc.
2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=146791703&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 146791703 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14754916 L6I jtl: Homeopathy issn: 14754916 maglogo: N pubinfo: dt: 2020 vid: 109 iid: 4 pid: 2811 pub: Thieme Medical Publishing Inc. place: New York, New York artinfo: ui: 146791703 146791703 146791703 10.1055/s-0040-1710044 146791703 ppf: 207 ppct: 6 formats: tig: atl: Preparation and Standardization of Escherichia coli Nosodes Sourced from Select E. coli Strains. aug: au: Munshi, Renuka Talele, Gitanjali Shah, Rajesh affil: Department of Clinical Pharmacology, TNMC & BYL Nair Ch. Hospital, Mumbai, Maharashtra, India sug: subj: Nosode Therapeutic Use Escherichia Coli Infections Drug Therapy Endotoxins Human Bacterial Contamination Microscopy Polymerase Chain Reaction Escherichia Coli Drug Resistance, Microbial Descriptive Statistics Catalase Indoles Urease ab: Background The nosodes are well-known preparations in homeopathy that are sourced from organisms and diseased materials. More than 40 known nosodes have been used in homeopathic practice for over a century. Having identified the need for scientifically developed new nosodes sourced from organisms that are currently prevalent, the preparation of Escherichia coli nosodes from different strains of the bacterium is presented in this article. Materials and Methods Escherichia coli strains (E. coli ATCC 11775E, ATCC 25922, and ATCC 8739) were identified, cultured, and tested for purity, and 20 billion cells were processed following the nosode preparation method given in the Homoeopathic Pharmacopoeia of India, group N1. Serial dilution and potentization for liquid potency were done up to 30c potency. Nosodes were prepared by two methods: from cell-free extract (endotoxin) and from entire-cell extract. Result Six nosodes were developed in total. Three univalent nosodes were prepared using individual endotoxins, one from each of the three E. coli strains; those three univalent nosodes were also combined as "Trivalent nosode-I". "Trivalent nosode-II" was prepared by mixing entire cells of the three E. coli strains. A mix of both Trivalent nosode-I and Trivalent nosode-II was labeled "EC-Polynosode". The safety profile of the potentized nosodes was documented by the non-detectability of traces of source material (absence of contamination, live organisms, or DNA material) through a culture test, sterility test, and molecular testing (polymerase chain reaction). Conclusion Different variants of E. coli nosodes were systematically and scientifically prepared and standardized using the cultures. Homeopathic pathogenetic trials, in-vitro efficacy studies, and clinical evaluation of E. coli nosodes (single, trivalent, or polyvalent nosodes) will be required in future. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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