Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing.
Translation of wound healing research is limited by the lack of an appropriate animal model, due to the anatomic and wound healing differences in animals and humans. Here, we characterize healing of grafted, full‐thickness human skin in an in vivo model of wound healing. Full‐thickness human skin, o...
| Publicado en: | Wound Repair & Regeneration Vol. 28; no. 6; pp. 812 - 823 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Nov2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=146915405&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 146915405 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov2020 vid: 28 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 146915405 145820299 146915405 146915405 10.1111/wrr.12847 146915405 ppf: 812 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing. aug: au: Karim, Aos S. Liu, Aiping Lin, Christie Uselmann, Adam J. Eliceiri, Kevin W. Brown, Matthew E. Gibson, Angela L. F. affil: Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison Wisconsin, USA sug: subj: Ischemia Neovascularization, Physiologic Wound Healing Physiology Xenografts Animal Studies Mice Models, Biological In Vivo Studies Anoxia ab: Translation of wound healing research is limited by the lack of an appropriate animal model, due to the anatomic and wound healing differences in animals and humans. Here, we characterize healing of grafted, full‐thickness human skin in an in vivo model of wound healing. Full‐thickness human skin, obtained from reconstructive operations, was grafted onto the dorsal flank of NOD.Cg‐KitW41JTyr + PrkdcscidIl2rgtm1Wjl/ThomJ mice. The xenografts were harvested 1 to 12 weeks after grafting, and histologic analyses were completed for viability, neovascularization, and hypoxia. Visual inspection of the xenograft shows drying and sloughing of the epidermis starting at week four. By week 12, the xenograft appears healed but has lost 63.05 ± 0.24% of the initial graft size. There is histologic evidence of epidermolysis as early as 2 weeks, which progresses until week 4, when new epidermis appears from the wound edges. Epidermal regeneration is complete by week 12, although the epidermis appears hypertrophied. An initial increase of infiltrating immune mouse cells into the xenograft normalizes to baseline 6 months after grafting. Neovascularization, as evidenced by positive staining for the proteins human CD31 and alpha smooth muscle actin, is present as early as 2 weeks after grafting at the interface between the xenograft and the mouse tissue. CD31 and alpha smooth muscle actin staining increased throughout the xenograft over the 12 weeks, leading to greater viability of the tissue. Likewise, there is increased Hypoxia Inducible Factor 1‐alpha expression at the interface of viable and nonviable tissue, which suggest a hypoxia‐driven process causing early graft loss. These findings illustrate human skin wound healing in an ischemic environment, providing a timeline for use of full thickness human skin after grafting in a murine model to study mechanisms underlying human skin wound healing. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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