Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.

Purpose: The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextr...

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Publicado en:European Journal of Nutrition Vol. 59; no. 8; pp. 3669 - 3690
Autores principales: Shinde, Tanvi, Perera, Agampodi Promoda, Vemuri, Ravichandra, Gondalia, Shakuntla V., Beale, David J., Karpe, Avinash V., Shastri, Sonia, Basheer, Waheedha, Southam, Benjamin, Eri, Rajaraman, Stanley, Roger
Formato: pictorial research tables/charts Journal Article
Publicado: Springer Nature Dec2020
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: European Journal of Nutrition
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      dt: Dec2020
      vid: 59
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00394-020-02200-9
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        atl: Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.
      aug:
        au:
          Shinde, Tanvi
          Perera, Agampodi Promoda
          Vemuri, Ravichandra
          Gondalia, Shakuntla V.
          Beale, David J.
          Karpe, Avinash V.
          Shastri, Sonia
          Basheer, Waheedha
          Southam, Benjamin
          Eri, Rajaraman
          Stanley, Roger
        affil: Centre for Food Safety and Innovation, Tasmanian Institute of Agriculture, University of Tasmania, 7250, Launceston, TAS, Australia
      sug:
        subj:
          Inflammatory Bowel Diseases Diet Therapy
          Prebiotics Pharmacodynamics
          Probiotics Pharmacodynamics
          Glucans Therapeutic Use
          Banana
          Bacillus
          Dietary Supplementation
          Drug Therapy, Combination
          Animal Studies
          Mice
          Colitis Diet Therapy
          Severity of Illness Indices
          Proteins
          Gene Expression Drug Effects
          Interleukins Drug Effects
          Interleukins Blood
          C-Reactive Protein Blood
          Fatty Acids Metabolism
      ab: Purpose: The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextran-sulfate sodium (DSS)-induced colitis in mice. Methods: Eight-week-old C57BL/6 mice were fed standard rodent chow diet supplemented with either B. coagulans, GBRS or its synbiotic combination. After 7 days supplementation, colitis was induced by adding 2% DSS in drinking water for 7 days while continuing the supplemented diets. Animal health was monitored and after 14 days all animals were sacrificed to measure the biochemical and histochemical changes associated with each supplement type. Results: The disease activity index and histological damage score for DSS-control mice (6.1, 17.1, respectively) were significantly higher (p < 0.0001) than the healthy mice. Synbiotic supplementation alleviated these markers (− 67%, − 94% respectively) more adequately than B. coagulans (− 52%, − 58% respectively) or GBRS (− 57%, − 26%, respectively) alone. Compared to DSS-control synbiotic supplementation significantly (p < 0.0001) maintained expressions of tight junction proteins. Moreover, synbiotic effects accounted for ~ 40% suppression of IL-1β and ~ 29% increase in IL-10 levels in serum while also reducing C-reactive protein (− 37%) compared to that of the DSS-control. While, B. coagulans alone could not induce additional levels of short-chain fatty acid (SCFA) production beyond the caecum, the synbiotic combination with GBRS resulted in substantial increased SCFA levels across the whole length of the colon. Conclusion: The synbiotic supplementation with B. coagulans and GBRS ameliorated the overall inflammatory status of the experimental IBD model via synergistic functioning. This supports researching its application in mitigating inflammation in human IBD.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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