Differences between muscle from osteoporotic and osteoarthritic subjects: in vitro study by diffusion-tensor MRI and histological findings.
Background: Osteoarthritis and osteoporosis are strongly coupled with alterations of muscles quality and fats metabolism. However, there are no studies for investigating possible differences between osteoporotic and osteoarthritic muscles. Understanding muscle-bone and muscle-cartilage interactions...
| Publicado en: | Aging Clinical & Experimental Research Vol. 32; no. 12; pp. 2489 - 2500 |
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| Autores principales: | , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147136218&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147136218 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15940667 2WMP jtl: Aging Clinical & Experimental Research issn: 15940667 maglogo: N pubinfo: dt: 2020 vid: 32 iid: 12 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 147136218 144171628 147136218 147136218 10.1007/s40520-020-01483-6 147136218 ppf: 2489 ppct: 11 formats: tig: atl: Differences between muscle from osteoporotic and osteoarthritic subjects: in vitro study by diffusion-tensor MRI and histological findings. aug: au: Di Pietro, Giulia Scimeca, Manuel Iundusi, Riccardo Celi, Monica Gasbarra, Elena Tarantino, Umberto Capuani, Silvia affil: Department of Orthopaedics and Traumatology, University of Rome Tor Vergata, "Policlinico Tor Vergata" Foundation, Viale Oxford, 81, 00133, Rome, Italy sug: subj: Osteoporosis Pathology Osteoarthritis Pathology Quadriceps Muscles Analysis Magnetic Resonance Spectroscopy Histological Techniques Human In Vitro Studies Female Quadriceps Muscles Metabolism Lipids Metabolism Muscle Fibers Analysis Comparative Studies One-Way Analysis of Variance Pearson's Correlation Coefficient Mitochondria Metabolism Female ab: Background: Osteoarthritis and osteoporosis are strongly coupled with alterations of muscles quality and fats metabolism. However, there are no studies for investigating possible differences between osteoporotic and osteoarthritic muscles. Understanding muscle-bone and muscle-cartilage interactions would be of high clinical value. Aim: Investigate potential microstructural and physiological differences between osteoporotic and osteoarthritic muscles by diffusion Nuclear Magnetic Resonance (NMR) imaging (diffusion MRI) and histological findings. Methods: Vastus-lateralis muscles excised from osteoporotic (n = 26, T Score < − 2.5, Kellgren–Lawrence ≤ 2) and osteoarthritic (n = 26, T Score > − 2.5, Kellgren-–Lawrence 3 and 4) age-matched women were investigated by NMR relaxometry, diffusion-tensor imaging (DTI) at 9.4 T, and histological techniques. Intramyocellular (IMCL) and extramyocellular (EMCL) lipid were quantified. The percentage and mean diameters of fibers I and II were evaluated. Relationship between mean diffusivity (MD), fractional anisotropy (FA), the DTI eigenvalues (λ1, λ2, λ3), histological findings in muscles and clinical data (Kellgren–Lawrence and T score, age, menopausal age, body mass index) were studied. Pairwise comparisons between groups were made using one-way analysis of variance and correlation between variables was assessed with linear correlation analysis (Pearson's r coefficient). Results: Osteoporotic muscles showed higher MD, λ1,λ2, λ3 compared to osteoarthritis ones. This is explainable with a significant higher density of IMCL droplets found inside the osteoarthritic muscles and a large amount of fibrotic tissue and IMCL infiltration between fibers, i.e. in endomysium and perimysium that lead to a more hindered diffusion. Furthermore, histological analysis suggests mitochondrial degeneration as the origin of the greatest amount of IMCL droplets in osteoarthritic muscles. Conclusion: This work highlights differences between muscles of osteoporotic and osteoarthritic subjects that can be quantified by NMR DTI investigations. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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