Assessment of a novel method to detect clarithromycin-resistant Helicobacter pylori using a stool antigen test reagent.
Background: The resistance rate of Helicobacter pylori to clarithromycin (CAM) is high among infected children in Japan. Therefore, a new method for detecting CAM-resistant H. pylori using a minimally invasive technique is strongly desired. We aimed to investigate the clinical usefulness of our newl...
| Publicado en: | BMC Gastroenterology Vol. 20; no. 1 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
BioMed Central
11/23/2020
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147156216&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147156216 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1471230X 1CHO jtl: BMC Gastroenterology issn: 1471230X maglogo: N pubinfo: dt: 11/23/2020 vid: 20 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 147156216 147156216 NLM33228552 10.1186/s12876-020-01549-9 NLM33228552 147156216 ppct: 1 formats: tig: atl: Assessment of a novel method to detect clarithromycin-resistant Helicobacter pylori using a stool antigen test reagent. aug: au: Kakiuchi, Toshihiko Hashiguchi, Kazutoshi Imamura, Ichiro Nakayama, Aiko Takamori, Ayako Okuda, Masumi Matsuo, Muneaki affil: Department of Pediatrics, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga-shi, 849-8501, Saga, Japan sug: subj: Helicobacter Pylori Drug Effects Helicobacter Pylori Helicobacter Infections Diagnosis Helicobacter Infections Drug Therapy Adult Japan Antibiotics Pharmacodynamics Microbial Culture and Sensitivity Tests Clarithromycin Pharmacodynamics Indicators and Reagents Adolescence Drug Resistance, Microbial Child Clinical Assessment Tools Scales Adult: 19-44 years Adolescent: 13-18 years Child: 6-12 years ab: Background: The resistance rate of Helicobacter pylori to clarithromycin (CAM) is high among infected children in Japan. Therefore, a new method for detecting CAM-resistant H. pylori using a minimally invasive technique is strongly desired. We aimed to investigate the clinical usefulness of our newly developed nested polymerase chain reaction-quenching probe (Nested PCR-QP) method using stool specimens.Methods: We first evaluated our method using a residual solution of the H. pylori stool antigen test for adolescents. Then, we evaluated our method using culture testing for adults.Results: Among 57 middle school students with H. pylori, the Nested PCR-QP test results of 53 (90.3%) were able to be analyzed. A total of 28 students had CAM resistance mutations. We found a genetic mutation in 28 students and no mutation in 23 students, and these results were consistent with those of PCR-direct sequencing. In the 23 adults who were diagnosed with H. pylori infection using the rapid urease test and culture testing, we were able to use Nested PCR-QP for analyzing 21 adults who tested positive in the stool H. pylori antigen test. The results obtained for all 21 adults were consistent with those obtained via the drug susceptibility test.Conclusions: Our novel method could be useful for non-invasively detecting CAM resistance mutations in H. pylori. This may help select a drug to reduce eradication failure rates against H. pylori. Trial registration This study was registered with the University Hospital Medical Information Network Clinical Trials Registry (no. UMIN000030632, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000034977 ) on 29 December 2017. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|