Mechanism of Action of Bu-Fei-Yi-Shen Formula in Treating Chronic Obstructive Pulmonary Disease Based on Network Pharmacology Analysis and Molecular Docking Validation.

Objective. To explore the mechanism of action of Bu-Fei-Yi-Shen formula (BFYSF) in treating chronic obstructive pulmonary disease (COPD) based on network pharmacology analysis and molecular docking validation. Methods. First of all, the pharmacologically active ingredients and corresponding targets...

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Published in:BioMed Research International pp. 1 - 13
Main Authors: Wu, Longchuan, Chen, Yu, Yi, Jiao, Zhuang, Yi, Cui, Lei, Ye, Chunhui
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell 11/27/2020
Online Access:View this record in EBSCOhost
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      dt: 11/27/2020
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        147247317
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        10.1155/2020/9105972
        147247317
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        atl: Mechanism of Action of Bu-Fei-Yi-Shen Formula in Treating Chronic Obstructive Pulmonary Disease Based on Network Pharmacology Analysis and Molecular Docking Validation.
      aug:
        au:
          Wu, Longchuan
          Chen, Yu
          Yi, Jiao
          Zhuang, Yi
          Cui, Lei
          Ye, Chunhui
        affil: Department of Respiratory Medicine, Huai'an Hospital of Traditional Chinese Medicine, Huai'an, 223001, China
      sug:
        subj:
          Pulmonary Disease, Chronic Obstructive Drug Therapy
          Molecular Docking Simulation
          Medicine, Chinese Traditional
          Plant Extracts Pharmacodynamics
          Plant Extracts Therapeutic Use
          Human
          Molecular Structure
          Interleukins
          Toll-Like Receptors
          Tumor Necrosis Factor
          Plants, Medicinal
      ab: Objective. To explore the mechanism of action of Bu-Fei-Yi-Shen formula (BFYSF) in treating chronic obstructive pulmonary disease (COPD) based on network pharmacology analysis and molecular docking validation. Methods. First of all, the pharmacologically active ingredients and corresponding targets in BFYSF were mined by the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database, the analysis platform, and literature review. Subsequently, the COPD-related targets (including the pathogenic targets and known therapeutic targets) were identified through the TTD, CTD, DisGeNet, and GeneCards databases. Thereafter, Cytoscape was employed to construct the candidate component-target network of BFYSF in the treatment of COPD. Moreover, the cytoHubba plug-in was utilized to calculate the topological parameters of nodes in the network; then, the core components and core targets of BFYSF in the treatment of COPD were extracted according to the degree value (greater than or equal to the median degree values for all nodes in the network) to construct the core network. Further, the Autodock vina software was adopted for molecular docking study on the core active ingredients and core targets, so as to verify the above-mentioned network pharmacology analysis results. Finally, the Omicshare database was applied in enrichment analysis of the biological functions of core targets and the involved signaling pathways. Results. In the core component-target network of BFYSF in treating COPD, there were 30 active ingredients and 37 core targets. Enrichment analysis suggested that these 37 core targets were mainly involved in the regulation of biological functions, such as response to biological and chemical stimuli, multiple cellular life processes, immunity, and metabolism. Besides, multiple pathways, including IL-17, Toll-like receptor (TLR), TNF, and HIF-1, played certain roles in the effect of BFYSF on treating COPD. Conclusion. BFYSF can treat COPD through the multicomponent, multitarget, and multipathway synergistic network, which provides basic data for intensively exploring the mechanism of action of BFYSF in treating COPD.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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