Combination of urease inhibitor and antiseptic inhibits urea decomposition‐induced ammonia production by Proteus mirabilis.
The decomposition of urea into ammonia by urease‐producing bacterium shows an elevation in the pH level, which can lead to incontinence‐associated dermatitis (IAD). This study aimed to examine the efficacy of a combination of antiseptic and urease inhibitor in inhibiting the decomposition of urea by...
| Publicado en: | International Wound Journal Vol. 17; no. 6; pp. 1558 - 1566 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Dec2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147597999&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147597999 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17424801 1725 jtl: International Wound Journal issn: 17424801 maglogo: Y pubinfo: dt: Dec2020 vid: 17 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 147597999 145898422 147597999 147597999 10.1111/iwj.13422 147597999 ppf: 1558 ppct: 8 formats: tig: atl: Combination of urease inhibitor and antiseptic inhibits urea decomposition‐induced ammonia production by Proteus mirabilis. aug: au: Mugita, Yuko Nakagami, Gojiro Minematsu, Takeo Kitamura, Aya Sanada, Hiromi affil: Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan sug: subj: Urease Antiinfective Agents, Local Pharmacodynamics Urea Metabolism Ammonia Drug Effects Proteus Treatment Outcomes Drug Therapy, Combination Human In Vitro Studies Microbial Culture and Sensitivity Tests Ammonia Blood Comparative Studies ab: The decomposition of urea into ammonia by urease‐producing bacterium shows an elevation in the pH level, which can lead to incontinence‐associated dermatitis (IAD). This study aimed to examine the efficacy of a combination of antiseptic and urease inhibitor in inhibiting the decomposition of urea by the urease‐producing bacterium Proteus mirabilis. We performed in vitro assays to compare the effects of a combination of antiseptic and urease inhibitor, antiseptic only, urease inhibitor only, and an untreated control with the effects of a urea‐containing solution. Cultured P. mirabilis was mixed with urea‐containing solution, followed by the addition of antiseptic and/or urease inhibitor. The main outcome used to assess the efficacy of the different treatments was ammonia concentration at 4‐hours post‐treatment initiation, and multiple comparison analysis was performed using Dunnett's test to compare the results between groups. Ammonia concentrations in samples treated with either antiseptic or urease inhibitor were lower than those in the untreated control, while the combination of antiseptic and urease inhibitor resulted in decreased ammonia concentrations compared with either treatment alone. Therefore, the application of both urease inhibitor and antiseptic is more effective for the inhibition of urea decomposition by urease‐producing bacteria. Novel preventive strategies using these reagents may be effective for preventing IAD. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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