Effects of two types of energy restriction on methylation levels of adiponectin receptor 1 and leptin receptor overlapping transcript in a mouse mammary tumour virus-transforming growth factor- α breast cancer mouse model.
The role of adiponectin and leptin signalling pathways has been suggested to play important roles in the protective effects of energy restriction (ER) on mammary tumour (MT) development. To study the effects of ER on the methylation levels in adiponectin receptor 1 (AdipoR1) and leptin receptor over...
| Published in: | British Journal of Nutrition Vol. 125; no. 1; pp. 1 - 10 |
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| Main Authors: | , , , , , , , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Cambridge University Press
1/14/2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147621623&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147621623 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00071145 1XX jtl: British Journal of Nutrition issn: 00071145 maglogo: N pubinfo: dt: 1/14/2020 vid: 125 iid: 1 pid: 15979 pub: Cambridge University Press artinfo: ui: 147621623 147621623 147621623 10.1017/S0007114519002757 147621623 ppf: 1 ppct: 9 formats: tig: atl: Effects of two types of energy restriction on methylation levels of adiponectin receptor 1 and leptin receptor overlapping transcript in a mouse mammary tumour virus-transforming growth factor- α breast cancer mouse model. aug: au: Cicekdal, M. B. Kazan, B. T. Tuna, B. G. Ozorhan, U. Ekici, I. D. Zhu, F. Suakar, O. Kuskucu, A. Bayrak, O. F. Arcaro, K. Cleary, M. P. Dogan, S. Atasayan, O. Yilmaz, B. affil: Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey sug: subj: Receptors, Cell Surface Metabolism Breast Neoplasms Epidermal Growth Factor Receptors Metabolism DNA Methylation Sequence Analysis Epigenomics Animal Studies Mice Comparative Studies ab: The role of adiponectin and leptin signalling pathways has been suggested to play important roles in the protective effects of energy restriction (ER) on mammary tumour (MT) development. To study the effects of ER on the methylation levels in adiponectin receptor 1 (AdipoR1) and leptin receptor overlapping transcript (Leprot) genes using the pyrosequencing method in mammary fat pad tissue, mouse mammary tumour virus-transforming growth factor-α (MMTV-TGF-α) female mice were randomly assigned to ad libitum (AL), chronic ER (CER, 15 % ER) or intermittent ER (3 weeks AL and 1 week 60 % ER in cyclic periods) groups at 10 weeks of age until 82 weeks of age. The methylation levels of AdipoR1 in the CER group were higher than those in the AL group at week 49/50 (P < 0·05), while the levels of methylation for AdipoR1 and Leprot genes were similar among the other groups. Also, the methylation levels at CpG2 and CpG3 regions of the promoter region of the AdipoR1 gene in the CER group were three times higher (P < 0·05), while CpG1 island of Leprot methylation was significantly lower compared with the other groups (P < 0·05). Adiponectin and leptin gene expression levels were consistent with the methylation levels. We also observed a change with ageing in methylation levels of these genes. These results indicate that different types of ER modify methylation levels of AdipoR1 and Leprot in different ways and CER had a more significant effect on methylation levels of both genes. Epigenetic regulation of these genes may play important roles in the preventive effects of ER against MT development and ageing processes. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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