miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.
Background. The asymptomatic onset, frequent recurrence, and poor prognosis of hepatocellular carcinoma (HCC) prompted us to identify new therapeutic targets or predictive markers of HCC diagnosis or prognosis. Methods. In this study, bioinformatics analysis was used to screen for target miRNAs from...
| Publicado en: | BioMed Research International pp. 1 - 17 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | pictorial research Journal Article |
| Publicado: |
Wiley-Blackwell
12/16/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147640765&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147640765 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/16/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 147640765 147640765 147640765 10.1155/2020/1964219 147640765 ppf: 1 ppct: 16 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma. aug: au: Chen, Kai Li, Zhonghu Zhang, Mengyun Wang, Bo Peng, Tao Shen, Yanbing Zhang, Jianxin Ye, Jiaxin Liu, Yu Tang, Di Peng, Minjie Ma, Dandan Xiao, Zhengkang Zhang, Yujun Jin, Weidong Li, Xiaowu affil: Hepatobiliary Surgery Institute, Southwest Hospital, Army Medical University, China sug: subj: MicroRNA Metabolism Carcinoma, Hepatocellular Physiopathology Neoplasm Metastasis Physiopathology Carcinoma, Hepatocellular Diagnosis Gene Expression Fibrosis Animal Studies Animals Mice Carcinoma, Hepatocellular Prognosis Bioinformatics Evaluation Blotting, Western Polymerase Chain Reaction Oncogenes In Vivo Studies Disease Progression ab: Background. The asymptomatic onset, frequent recurrence, and poor prognosis of hepatocellular carcinoma (HCC) prompted us to identify new therapeutic targets or predictive markers of HCC diagnosis or prognosis. Methods. In this study, bioinformatics analysis was used to screen for target miRNAs from the open-access TCGA database. Transwell assays, Western blotting, and qRT-PCR analyses were used to detect cellular functions and gene expression in HCC cells and samples. A nude mouse tumorigenesis model was established to facilitate the observation of HCC progression. Other assays included luciferase reporter assays, IHC, and survival analysis. Results. We found that the identified miR-876 from TCGA was expressed at low levels in HCC cell lines and that low miR-876 expression was corrected with liver cirrhosis, tumor thrombus, and TNM stage. Further research revealed that miR-876 regulated cell invasion, EMT, and collagen expression by targeting POSTN expression. miR-876 and POSTN were inversely correlated in HCC samples and associated with EMT status and liver fibrosis in clinical HCC tissues. miR-876 inhibited the liver cancer progression in in vivo animal assays. Finally, both miR-876 and POSTN were risk factors for HCC survival, and HCC patients with combined low miR-876 and high POSTN expression had worse prognosis. Conclusions. miR-876 inhibited HCC EMT and fibrosis by targeting POSTN, thus affecting HCC progression and prognosis. miR-876 and POSTN may be useful therapeutic targets or prognostic markers of HCC. pubtype: Academic Journal doctype: pictorial research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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