The Restorative Effect of Apocynin and Catalase in L-Arginine-Induced Hypotension on Normotensive Subjects -- the Role of Oxidative Stress.
L-arginine is a substrate for nitric oxide synthase (NOS) responsible for the production of NO. This investigation studied the effect of apocynin, an NADPH oxidase inhibitor and catalase, an H2O2 scavenger on L-arginine-induced oxidative stress and hypotension. Forty Wistar-Kyoto rats were treated f...
| Publicado en: | Physiological Research Vol. 69; no. 6; pp. 1051 - 1067 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Journal Article |
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Institute of Physiology, Academy of Sciences of the Czech Republic
Dec2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=147980045&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 147980045 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 08628408 1CVW jtl: Physiological Research issn: 08628408 maglogo: N pubinfo: dt: Dec2020 vid: 69 iid: 6 pid: 24634 pub: Institute of Physiology, Academy of Sciences of the Czech Republic artinfo: ui: 147980045 10.33549/physiolres.934426 147980045 ppf: 1051 ppct: 16 formats: fmt: @attributes: type: P tig: atl: The Restorative Effect of Apocynin and Catalase in L-Arginine-Induced Hypotension on Normotensive Subjects -- the Role of Oxidative Stress. aug: au: Tan Yong CHIA MURUGAIYAH, Vikneswaran SATTAR, Munavvar Abdul KARIM KHAN, Nurzalina Abdul AHMAD, Ashfaq ABDULLA, Mohammed Hadi JOHNS, Edward James Ho Yoke MEI AKHTAR, Safia AHMAD, Fiaz Uddin affil: Cardiovascular and Renal Physiology Research Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia sug: ab: L-arginine is a substrate for nitric oxide synthase (NOS) responsible for the production of NO. This investigation studied the effect of apocynin, an NADPH oxidase inhibitor and catalase, an H2O2 scavenger on L-arginine-induced oxidative stress and hypotension. Forty Wistar-Kyoto rats were treated for 14 days with vehicle, L-arginine (12.5 mg/ml p.o.), L-arginine + apocynin (2.5 mmol/l p.o.), L-arginine + catalase (10000 U/kg/day i.p.) and L-arginine plus apocynin + catalase respectively. Weekly renal functional and hemodynamic parameters were measured and kidneys harvested at the end of the study for histopathological and renal NADPH oxidase 4 (Nox4) assessments. L-arginine administration in normotensive rats decreased systolic blood pressure (120±2 vs. 91±2 mmHg) and heart rate (298±21 vs. 254±15 bpm), enhanced urinary output (21.5±4.2 vs. 32±1.9 ml/24 h, increased creatinine clearance (1.72±0.56 vs. 2.62±0.40 ml/min/kg), and fractional sodium excretion (0.88±0.16 vs. 1.18±0.16 %), caused proteinuria (28.10±1.93 vs. 35.26±1.69 mg/kg/day) and a significant decrease in renal cortical blood perfusion (292±3 vs. 258±5 bpu) and pulse wave velocity (3.72±0.20 vs 2.84±0.13 m/s) (all P<0.05). L-arginine increased plasma malondialdehyde (by ~206 % P<0.05) and NO (by ~51 %, P<0.05) but decreased superoxide dismutase (by ~31 %, P<0.05) and total antioxidant capacity (by ---35 %, P<0.05) compared to control. Renal Nox4 mRNA activity was approximately 2.1 fold higher (P<0.05) in the L-arginine-treated rats but was normalized by apocynin and apocynin plus catalase treatment. Administration of apocynin and catalase, but not catalase alone to rats fed L-arginine, restored the deranged renal function and structure, prevented hypotension and enhanced the antioxidant capacity and suppressed Nox4 expression. These findings suggest that apocynin and catalase might be used prophylactically in the states of oxidative stress. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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