DWI and IVIM are predictors of Ki67 proliferation index: direct comparison of MRI images and pathological slices in a murine model of rhabdomyosarcoma.
Objectives: The purpose of this study was to investigate the correlation of diffusion-weighted imaging (DWI) and intravoxel incoherent motion (IVIM) with the Ki67 proliferation index in a murine model of rhabdomyosarcoma.Methods: The rhabdomyosarcoma model was established by injecting RD cells into...
| Publicado en: | European Radiology Vol. 30; no. 3; pp. 1334 - 1342 |
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| Autores principales: | , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=148390821&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 148390821 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: 2020 vid: 30 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 148390821 148390821 NLM31705255 148390821 10.1007/s00330-019-06509-w NLM31705255 148390821 ppf: 1334 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: DWI and IVIM are predictors of Ki67 proliferation index: direct comparison of MRI images and pathological slices in a murine model of rhabdomyosarcoma. aug: au: Yuan, Yuan Zeng, Dewei Liu, Yajie Tao, Juan Zhang, Yu Yang, Jie Lkhagvadorj, Tsendjav Yin, Zhenzhen Wang, Shaowu affil: Department of Radiology, The Second Hospital of Dalian Medical University, Dalian, China sug: subj: Cell Physiology Tumor Markers, Biological Metabolism Rhabdomyosarcoma Animals Female Magnetic Resonance Imaging Methods Mice Rhabdomyosarcoma Pathology Rhabdomyosarcoma Metabolism Models, Biological Specialties, Medical Magnetic Resonance Imaging Immunohistochemistry Human Funding Source Female ab: Objectives: The purpose of this study was to investigate the correlation of diffusion-weighted imaging (DWI) and intravoxel incoherent motion (IVIM) with the Ki67 proliferation index in a murine model of rhabdomyosarcoma.Methods: The rhabdomyosarcoma model was established by injecting RD cells into the right hind flank of nude mice. The mice underwent 3.0T magnetic resonance imaging (MRI), including DWI and IVIM. The apparent diffusion coefficient (ADC), D, D*, and f were calculated with the ADW4.7 workstation. A specialized method was employed to ensure the pathological sections corresponded to the correct MRI slices. The Ki67 proliferation index was analyzed by immunohistochemistry, and any possible correlations were assessed between the DWI and IVIM parameters and Ki67 proliferation index.Results: Twenty-seven rhabdomyosarcoma mice were established successfully. After 46 days, the average tumor volume reached 1094.78 ± 678.77 mm3. The average ADC, D, and D* values were 1.0470 ± 0.2036 × 10-3 mm2/s, 0.7237 ± 0.0971 × 10-3 mm2/s, and 4.8497 ± 1.6293 × 10-3 mm2/s, respectively. The range in f values was 0.102-0.229. The ADC and D values showed a moderate negative correlation with the Ki67 proliferation indexes (r = - 0.543, p = 0.003; r = - 0.491, p = 0.009, respectively). In addition, the f value showed a weak negative correlation with the Ki67 proliferation indexes (r = - 0.151, p = 0.451), while the D* value showed no association with the Ki67 proliferation indexes (r = - 0.037, p = 0.853).Conclusions: The ADC value of DWI, along with the D value of IVIM, may be reflective of Ki67 proliferation indexes in murine models of rhabdomyosarcoma.Key Points: • DWI and IVIM parameters are correlated with Ki67 proliferation indexes in rhabdomyosarcoma mouse models. • A specialized method ensured a strong correlation between pathological sections and MRI slices, resulting in a robust radiological-pathological correlation. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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