Novel Real-time Digital Pressure Sensor Reveals Wide Variations in Current Nerve Crush Injury Models.
Introduction: Peripheral nerve crush injury (PNCI) models are commonly used to study nerve damage and the potential beneficial effects of novel therapeutic strategies. Current models of PNCI rely on inter-device and operator precision to limit the variation with applied pressure. Although the inabil...
| Published in: | Military Medicine Vol. 186; pp. 473 - 479 |
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| Main Authors: | , , , , , |
| Format: | research Journal Article |
| Published: |
Oxford University Press / USA
2021 Supplement
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=148590392&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 148590392 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00264075 4DV jtl: Military Medicine issn: 00264075 maglogo: N pubinfo: dt: 2021 Supplement vid: 186 pid: 622 pub: Oxford University Press / USA artinfo: ui: 148590392 148590392 NLM33499447 148590392 10.1093/milmed/usaa346 NLM33499447 148590392 ppf: 473 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Novel Real-time Digital Pressure Sensor Reveals Wide Variations in Current Nerve Crush Injury Models. aug: au: Wandling, Grant D. Jung Il Lee Talukder, M. A. Hassan Govindappa, Prem Kumar Elfar, John C. Lee, Jung Il affil: Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey, PA 17033, USA. sug: subj: Denervation Mice Female Peripheral Nerves Injuries Animal Studies Reproducibility of Results Comparative Studies Multicenter Studies Evaluation Research Validation Studies Funding Source Female ab: Introduction: Peripheral nerve crush injury (PNCI) models are commonly used to study nerve damage and the potential beneficial effects of novel therapeutic strategies. Current models of PNCI rely on inter-device and operator precision to limit the variation with applied pressure. Although the inability to accurately quantify the PNCI pressure may result in reduced reproducibility between animals and studies, there is very limited information on the standardization and quantification of applied pressure with PNCI. To address this deficit, we constructed a novel device comprised of an Arduino UNO microcontroller board and Force Sensitive Resistor capable of reporting the real-time pressure applied to a nerve.Methods: Two forceps and two needle drivers were used to perform 30-second PNCIs to the sciatic nerves of mice (n = 5/group). Needle drivers were set to the first notch, and a jig was used to hold the forceps pinch at a reproducible pressure. The Force Sensitive Resistor was interposed in-series between the nerve and instrument during PNCI.Results: Data collected from these procedures displayed average needle driver pressures an order of multitude greater than forceps pressures. Additionally, needle driver inter- and intra-procedure pressure remained more consistent than forceps pressure, with needle driver coefficient of variation equal to 14.5% vs. a forceps coefficient of variation equal to 45.4%.Conclusions: This is the first demonstration of real-time pressure measurements in PNCI models and it reveals that the applied pressures are dependent on the types of device used. The large disparity in pressure represents an inability to apply graded accurate and consistent intermediate pressure gradients in PNCI. These findings indicate a need for documentation of pressure severity as a screening for PNCI in animals, and the real-time pressure sensor could be a useful tool in monitoring and applying consistent pressure, reducing the outcome variability within the same experimental model of PNCI. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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