A Simple Panel of IDH1 and P53 in Differential Diagnosis Between Low-Grade Astrocytoma and Reactive Gliosis.
Background: Reactive gliosis is a response of glial tissue to different types of injury such as brain abscess, trauma, hemorrhage, or even neoplastic process. In some circumstances, especially when the tissue biopsy is small, there may be difficulty to discriminate this reactive condition with low-g...
| Publicado en: | Clinical Pathology Vol. 14; pp. 1 - 5 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
2/11/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=148704857&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 148704857 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 2632010X M8A9 jtl: Clinical Pathology issn: 2632010X maglogo: Y pubinfo: dt: 2/11/2021 vid: 14 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 148704857 148704857 148704857 10.1177/2632010X20986168 148704857 ppf: 1 ppct: 4 formats: fmt: @attributes: type: P tig: atl: A Simple Panel of IDH1 and P53 in Differential Diagnosis Between Low-Grade Astrocytoma and Reactive Gliosis. aug: au: Geramizadeh, Bita Kohandel-Shirazi, Mahsa Soltani, Ahmad affil: Department of Pathology, Medical School of Shiraz University, Shiraz University of Medical Sciences, Shiraz, Iran sug: subj: Glioma Diagnosis Brain Diseases Diagnosis Pathologic Processes Diagnosis Diagnosis, Differential Oxidoreductases Analysis Proteins Analysis Mutation Immunohistochemistry Methods Predictive Value of Tests Evaluation Human Child, Preschool Child Adolescence Adult Middle Age Aged Record Review Sensitivity and Specificity Proteins Metabolism Staining and Labeling Methods Funding Source Child, Preschool: 2-5 years Child: 6-12 years Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years ab: Background: Reactive gliosis is a response of glial tissue to different types of injury such as brain abscess, trauma, hemorrhage, or even neoplastic process. In some circumstances, especially when the tissue biopsy is small, there may be difficulty to discriminate this reactive condition with low-grade diffuse astrocytoma (World Health Organization [WHO] grade II) by conventional hematoxylin and eosin (H&E) slides, so some immunohistochemical and molecular markers have been introduced for this differential diagnosis. One of the important aspects of updated WHO classification in 2016 has been dividing some of the glial tumor according to IDH1 (isocitrate dehydrogenase 1) mutation. Objectives: In this study, we tried to evaluate IDH1 and P53 mutation by immunohistochemistry as a simple and highly specific and sensitive method to differentiate low-grade astrocytoma and reactive gliosis. Material and methods: For 5 years (2013-2018), 50 cases of clinically documented reactive gliosis and 50 cases of low-grade astrocytoma were evaluated for the presence or absence of IDH1 and P53 mutation by immunohistochemistry. Results: Isocitrate dehydrogenase 1 was positive in 92% and 4% of the astrocytoma and reactive gliosis cases and P53 was positive in 90% and 4% of the cases with the final diagnosis of astrocytoma and reactive gliosis, respectively. Discussion and conclusion: Combination of P53 and IDH1 as an immunohistochemical panel showed specificity of 96% and sensitivity of 91% for differential diagnosis of reactive gliosis and low-grade astrocytoma. These 2 markers can be extremely helpful for this differential diagnosis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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