Passive Auto Focusing of Pathological Microscope with Intelligent Field Image Collection Mechanism.

The microscope is one of the widely used pathological equipment to analyze body fluids like blood, sputum, etc. in granular level. In order to reduce workload on pathologists and strengthen the telehealth services, an automatic self-focusing microscope with different field image collection mechanism...

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Published in:Journal of Medical Systems Vol. 45; no. 2; pp. 1 - 16
Main Authors: Ghosh, Pramit, Bhattacharjee, Debotosh, Nasipuri, Mita
Format: algorithm equations & formulas pictorial tables/charts Journal Article
Published: Springer Nature 2021
Online Access:View this record in EBSCOhost
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      dt: 2021
      vid: 45
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      pub: Springer Nature
      place: New York, New York
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        148904079
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        10.1007/s10916-020-01688-2
        148904079
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        atl: Passive Auto Focusing of Pathological Microscope with Intelligent Field Image Collection Mechanism.
      aug:
        au:
          Ghosh, Pramit
          Bhattacharjee, Debotosh
          Nasipuri, Mita
        affil: Department of Computer Science and Engineering, RCC Institute of Information Technology, 700015, Kolkata, India
      sug:
        subj:
          Microscopy Methods
          Autoanalyzers
          Technology, Medical
          Digital Imaging Methods
          Automation, Laboratory
          Body Fluids Analysis
          Laboratory Equipment and Supplies
          Pathologists
          Telehealth Trends
      ab: The microscope is one of the widely used pathological equipment to analyze body fluids like blood, sputum, etc. in granular level. In order to reduce workload on pathologists and strengthen the telehealth services, an automatic self-focusing microscope with different field image collection mechanism is required. In this work, the conversion of a compound microscope into a complete digital self-focusing automatic microscope, with intelligent field image collection mechanism, is discussed. This method uses passive autofocusing technique. In this method, most informative regions are identified on the basis of texture information. Features from these identified regions are used for autofocusing the microscope. This system is capable of collecting multiple snaps from different regions of the smear sample slides. The problem with the smear slide is that it has un-uniform thickness upon the glass slide. So some region has a very thick layer and some region has a very thin layer. In general, both of these regions are not considered for pathological analysis. The proposed system is capable to detect the region of smear slide which is suitable for collection of snap images. A soft computing approach is used to detect the desired regions of the sample in the slide. The Raspberry pi is used to design the control section. Multi-threaded parallel programming is used to optimize I/O execution and waiting time. The performance of the proposed system is satisfactory. The average peak signal-to-noise ratio (PSNR) is about 33 in comparison with manual focusing by the domain expert. The performance of the system in terms of computation time, which is calculated on the benchmark microscopic image dataset, is better than other learning-based methods. Autofocusing of pathological microscope with an intelligent field image collection mechanism is highly useful in the remote healthcare domain. This work basically describes a mechanism to migrate the conventional compound microscope into a tale-health service compatible (IoT enabled) microscope. This system is highly suitable for developing countries where an overall change of existing infrastructure is difficult due to economic reasons.
      pubtype: Academic Journal
      doctype:
        algorithm
        equations & formulas
        pictorial
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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