Trehalose as quantitative biomarker for in vivo diagnosis and treatment follow-up in cryptococcomas.
Brain lesions caused by Cryptococcus neoformans or C. gattii (cryptococcomas) are typically difficult to diagnose correctly and treat effectively, but rapid differential diagnosis and treatment initiation are crucial for good outcomes. In previous studies, cultured cryptococcal isolates and ex vivo...
| Publicado en: | Translational Research: The Journal of Laboratory & Clinical Medicine Vol. 230; pp. 111 - 123 |
|---|---|
| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Apr2021
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=149013624&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 149013624 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 19315244 2Q66 jtl: Translational Research: The Journal of Laboratory & Clinical Medicine issn: 19315244 maglogo: N pubinfo: dt: Apr2021 vid: 230 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 149013624 149013624 NLM33166695 149013624 10.1016/j.trsl.2020.11.001 NLM33166695 149013624 ppf: 111 ppct: 12 formats: tig: atl: Trehalose as quantitative biomarker for in vivo diagnosis and treatment follow-up in cryptococcomas. aug: au: Vanherp, Liesbeth Poelmans, Jennifer Weerasekera, Akila Hillen, Amy Croitor-Sava, Anca R Sorrell, Tania C Lagrou, Katrien Vande Velde, Greetje Himmelreich, Uwe affil: Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium sug: subj: Disaccharides Analysis Meningitis, Cryptococcal Diagnosis Meningitis, Cryptococcal Cerebrospinal Fluid Cryptococcus Drug Effects Human Animal Studies Mice Meningitis, Cryptococcal Blood Amphotericin B Pharmacodynamics Disaccharides Cerebrospinal Fluid Disaccharides Blood Drug Combinations Fluconazole Pharmacodynamics Meningitis, Cryptococcal Pathology Bile Acids and Salts Pharmacodynamics Female Cryptococcus Metabolism Middle Age Comparative Studies Multicenter Studies Evaluation Research Validation Studies Middle Aged: 45-64 years Female ab: Brain lesions caused by Cryptococcus neoformans or C. gattii (cryptococcomas) are typically difficult to diagnose correctly and treat effectively, but rapid differential diagnosis and treatment initiation are crucial for good outcomes. In previous studies, cultured cryptococcal isolates and ex vivo lesion material contained high concentrations of the virulence factor and fungal metabolite trehalose. Here, we studied the in vivo metabolic profile of cryptococcomas in the brain using magnetic resonance spectroscopy (MRS) and assessed the relationship between trehalose concentration, fungal burden, and treatment response in order to validate its suitability as marker for early and noninvasive diagnosis and its potential to monitor treatment in vivo. We investigated the metabolites present in early and late stage cryptococcomas using in vivo 1H MRS in a murine model and evaluated changes in trehalose concentrations induced by disease progression and antifungal treatment. Animal data were compared to 1H and 13C MR spectra of Cryptococcus cultures and in vivo data from 2 patients with cryptococcomas in the brain. In vivo MRS allowed the noninvasive detection of high concentrations of trehalose in cryptococcomas and showed a comparable metabolic profile of cryptococcomas in the murine model and human cases. Trehalose concentrations correlated strongly with the fungal burden. Treatment studies in cultures and animal models showed that trehalose concentrations decrease following exposure to effective antifungal therapy. Although further cases need to be studied for clinical validation, this translational study indicates that the noninvasive MRS-based detection of trehalose is a promising marker for diagnosis and therapeutic follow-up of cryptococcomas. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|