In vivo calibration of the T2* cardiovascular magnetic resonance method at 1.5 T for estimation of cardiac iron in a minipig model of transfusional iron overload.
Background: Non-invasive estimation of the cardiac iron concentration (CIC) by T2* cardiovascular magnetic resonance (CMR) has been validated repeatedly and is in widespread clinical use. However, calibration data are limited, and mostly from post-mortem studies. In the present study, we performed a...
| Publicado en: | Journal of Cardiovascular Magnetic Resonance (Elsevier B.V. ) Vol. 23; no. 1; pp. 1 - 17 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Elsevier B.V.
3/11/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=149171934&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 149171934 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1532429X 86WS jtl: Journal of Cardiovascular Magnetic Resonance (Elsevier B.V. ) issn: 1532429X maglogo: N pubinfo: dt: 3/11/2021 vid: 23 iid: 1 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 149171934 149171934 149171934 10.1186/s12968-021-00715-6 149171934 ppf: 1 ppct: 16 formats: tig: atl: In vivo calibration of the T2* cardiovascular magnetic resonance method at 1.5 T for estimation of cardiac iron in a minipig model of transfusional iron overload. aug: au: Jensen, Peter Diedrich Nielsen, Asbjørn Haaning Simonsen, Carsten Wiberg Baandrup, Ulrik Thorngren Jensen, Svend Eggert Bøgsted, Martin Magnusdottir, Sigridur Olga Jensen, Anne Birthe Helweg Kjaergaard, Benedict affil: Department of Hematology, Aalborg University Hospital, PO box 365, 9100, Aalborg, Denmark sug: subj: Magnetic Resonance Imaging Methods Iron Blood Iron Overload Blood Transfusion Animal Studies Models, Biological In Vivo Studies Swine Spectrum Analysis Biopsy Magnetic Resonance Spectroscopy Spearman's Rank Correlation Coefficient Regression Heart Descriptive Statistics ab: Background: Non-invasive estimation of the cardiac iron concentration (CIC) by T2* cardiovascular magnetic resonance (CMR) has been validated repeatedly and is in widespread clinical use. However, calibration data are limited, and mostly from post-mortem studies. In the present study, we performed an in vivo calibration in a dextran-iron loaded minipig model. Methods: R2* (= 1/T2*) was assessed in vivo by 1.5 T CMR in the cardiac septum. Chemical CIC was assessed by inductively coupled plasma-optical emission spectroscopy in endomyocardial catheter biopsies (EMBs) from cardiac septum taken during follow up of 11 minipigs on dextran-iron loading, and also in full-wall biopsies from cardiac septum, taken post-mortem in another 16 minipigs, after completed iron loading. Results: A strong correlation could be demonstrated between chemical CIC in 55 EMBs and parallel cardiac T2* (Spearman rank correlation coefficient 0.72, P < 0.001). Regression analysis led to [CIC] = (R2* − 17.16)/41.12 for the calibration equation with CIC in mg/g dry weight and R2* in Hz. An even stronger correlation was found, when chemical CIC was measured by full-wall biopsies from cardiac septum, taken immediately after euthanasia, in connection with the last CMR session after finished iron loading (Spearman rank correlation coefficient 0.95 (P < 0.001). Regression analysis led to the calibration equation [CIC] = (R2* − 17.2)/31.8. Conclusions: Calibration of cardiac T2* by EMBs is possible in the minipig model but is less accurate than by full-wall biopsies. Likely explanations are sampling error, variable content of non-iron containing tissue and smaller biopsies, when using catheter biopsies. The results further validate the CMR T2* technique for estimation of cardiac iron in conditions with iron overload and add to the limited calibration data published earlier. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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