LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion.
Purpose. Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. Methods. The expression of RBM5-AS1 i...
| Publicado en: | BioMed Research International pp. 1 - 10 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
3/16/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=149314930&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 149314930 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/16/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 149314930 149314930 149314930 10.1155/2021/5271291 149314930 ppf: 1 ppct: 9 formats: fmt: @attributes: type: P tig: atl: LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion. aug: au: Deng, Biyong Pan, Runsang Ou, Xin Wang, Taizhe Wang, Weiguo Nie, Yingjie Chen, Houping affil: The Orthopedic Surgery Department of Guizhou Province Orthopedic Hospital, China sug: subj: Osteosarcoma Drug Therapy Cell Proliferation Drug Effects Cell Movement Drug Effects RNA-Binding Proteins Pharmacodynamics Mice Animal Studies Polymerase Chain Reaction Gene Expression Cell Line, Tumor Drug Effects Flow Cytometry Blotting, Western In Vitro Studies In Vivo Studies ab: Purpose. Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. Methods. The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5. Results. LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells. Conclusion. To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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