Comparative transcriptome analysis of transcripts of uncertain coding potential in septic myocardial depression.
Background: Septic shock with myocardial depression is very common in intensive care units. However, the exact molecular mechanisms underlying sepsis-induced myocardial depression remain unclear. Whether the profiles of transcripts of uncertain coding potential (TUCPs) differ between patients with a...
| Published in: | BMC Cardiovascular Disorders Vol. 21; no. 1; pp. 1 - 10 |
|---|---|
| Main Authors: | , , , |
| Format: | research Journal Article |
| Published: |
BioMed Central
4/8/2021
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=149713567&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 149713567 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712261 1CHA jtl: BMC Cardiovascular Disorders issn: 14712261 maglogo: N pubinfo: dt: 4/8/2021 vid: 21 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 149713567 149713567 NLM33832434 149713567 10.1186/s12872-021-01973-z NLM33832434 149713567 ppf: 1 ppct: 9 formats: tig: atl: Comparative transcriptome analysis of transcripts of uncertain coding potential in septic myocardial depression. aug: au: Zhang, Tie-Ning Wen, Ri Yang, Ni Liu, Chun-Feng affil: Department of Pediatrics, PICU, Shengjing Hospital of China Medical University, No. 36, SanHao Street, 110004, Shenyang City, Liaoning Province, People's Republic of China sug: subj: Shock, Septic Myocardium Metabolism Heart Diseases Gene Expression Profiling Male Animal Studies Heart Diseases Physiopathology Shock, Septic Physiopathology Signal Transduction Shock, Septic Complications Heart Diseases Metabolism Heart Diseases Etiology Shock, Septic Metabolism Rats Molecular Structure Models, Biological Comparative Studies Multicenter Studies Evaluation Research Validation Studies Clinical Assessment Tools Male ab: Background: Septic shock with myocardial depression is very common in intensive care units. However, the exact molecular mechanisms underlying sepsis-induced myocardial depression remain unclear. Whether the profiles of transcripts of uncertain coding potential (TUCPs) differ between patients with and without myocardial depression is also unknown. Our study aimed to find expression differences between groups of TUCPs and determine their potential functions in a preclinical model.Methods: We generated rat models of hypodynamic septic shock induced by lipopolysaccharide. A total of 12 rats were established and left ventricular tissue from each was collected. We performed RNA-seq to identify TUCPs in each sample. Transcripts with an corrected P value of < 0.05 were defined as differentially expressed (DE). We also performed GO terms and KEGG analysis to identify the potential functions of DE TUCPs.Results: A total of 4,851 TUCPs were identified in heart samples, 85 of which were expressed differently between the sepsis and control groups. Further bioinformatic analyses suggested that TUCPs play important roles in myocardial contraction, energy regulation, and metabolic processes, and are also involved in the regulation of several pathways.Conclusion: Our results demonstrate that TUCPs both participate in and mediate the pathological process of myocardial depression. Our study improves the understanding of the basic molecular mechanisms underlying myocardial depression from a novel perspective. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|