Novel preclinical method for evaluating the efficacy of a percutaneous treatment in human ex vivo calcified plaque.

The lack of suitable atherosclerotic calcification models and testing strategies inhibits preclinical efficacy testing of existing and novel percutaneous devices. The goal of this study is to develop a preclinical testing method for quantitatively and qualitatively evaluating the efficacy of noncomp...

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Published in:Medical & Biological Engineering & Computing Vol. 59; no. 4; pp. 799 - 812
Main Authors: Chisena, Robert S., Sengenberger, Jordan, Shih, Albert J., Gurm, Hitinder
Format: Journal Article
Published: Springer Nature Apr2021
Online Access:View this record in EBSCOhost
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      dt: Apr2021
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      pub: Springer Nature
      place: New York, New York
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        atl: Novel preclinical method for evaluating the efficacy of a percutaneous treatment in human ex vivo calcified plaque.
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        au:
          Chisena, Robert S.
          Sengenberger, Jordan
          Shih, Albert J.
          Gurm, Hitinder
        affil: Department of Mechanical Engineering, University of Michigan at Ann Arbor, 2350 Hayward St, 48109, Ann Arbor, MI, USA
      sug:
        subj:
          Atherosclerosis
          Angioplasty, Transluminal, Percutaneous Coronary
          Atherosclerosis Therapy
          Tomography, X-Ray Computed
          Lower Extremity
      ab: The lack of suitable atherosclerotic calcification models and testing strategies inhibits preclinical efficacy testing of existing and novel percutaneous devices. The goal of this study is to develop a preclinical testing method for quantitatively and qualitatively evaluating the efficacy of noncompliant balloon angioplasty (NC BA) treatment in human ex vivo calcified plaque (CP). NC BA using a 3- and 4-mm diameter balloon was performed on an ex vivo tibial calcified vessel obtained from an amputation. Three-dimensional microcomputed tomography (μ-CT) imaging was performed pre- and post-BA to compare crack density in the CP. Comparing the pre- and posttreatment three-dimensional μ-CT images showed a glass-like cracking that occurred in the CP due to the BA procedure. Expansion of the 3-mm balloon showed little tissue deformation and no CP cracking. Although expansion of the 4-mm balloon occurred nonuniformly along balloon length and across the perpendicular projections, the balloon generated cracking throughout the CP, which allowed the surrounding elastic tissue to be dilated. This combined X-ray microscopy and μ-CT technique is a useful preclinical tool for quantifying the efficacy of percutaneous treatments for CP. Because of its nondestructive nature, the CP structure can be visualized pre- and posttreatment to determine the treatment effect.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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