Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy Ducks.
Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, n = 5) and lowest (L group, n = 5) body weight w...
| Publicado en: | BioMed Research International pp. 1 - 10 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
4/19/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=149888063&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 149888063 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 4/19/2021 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 149888063 149888063 149888063 10.1155/2021/6648435 149888063 ppf: 1 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy Ducks. aug: au: Wang, Xingxin Xiao, Yingping Yang, Hua Lu, Lizhi Liu, Xiuting Lyu, Wentao affil: College of Animal Science, ZheJiang A&F University, Hangzhou, China sug: subj: Gene Expression Profiling Genes Poultry Metabolism Birds Ileum Metabolism Human Descriptive Statistics Body Weight Ileum RNA Analysis Health Screening Ontologies Genome Glutathione Metabolism Proteins Digestion Polymerase Chain Reaction Gene Expression ab: Muscovy ducks are among the best meat ducks in the world. The objective of this study was to identify genes related to growth metabolism through transcriptome analysis of the ileal tissue of Muscovy ducks. Duck ileum samples with the highest (H group, n = 5) and lowest (L group, n = 5) body weight were selected from two hundred 70-day-old Muscovy ducks for transcriptome analysis by RNA sequencing. In the screening of differentially expressed genes (DEGs) between the H and L groups, a total of 602 DEGs with a fold change no less than 2 were identified, among which 285 were upregulated and 317 were downregulated. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that glutathione metabolism, pyrimidine metabolism, and protein digestion and absorption processes played a vital role in regulating growth and metabolism. The results showed that 7 genes related to growth and metabolism, namely, ANPEP, ENPEP, UPP1, SLC2A2, SLC6A19, NME4, and LOC106034733, were significantly expressed in group H, which was consistent with the phenotype results. The validation of these 7 genes using real-time quantitative PCR results indicated that the expression level of ENPEP was significantly different between the H and L groups (P < 0.05). This study provides a theoretical basis for exploring the influence of the ileum on growth and metabolism in ducks. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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