Alcohol‐associated intestinal dysbiosis alters mucosal‐associated invariant T‐cell phenotype and function.
Background: Chronic alcohol consumption is associated with a compromised innate and adaptive immune responses to infectious disease. Mucosa‐associated invariant T (MAIT) cells play a critical role in antibacterial host defense. However, whether alcohol‐associated deficits in innate and adaptive immu...
| Published in: | Alcoholism: Clinical & Experimental Research Vol. 45; no. 5; pp. 934 - 948 |
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| Main Authors: | , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
May2021
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=150368893&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 150368893 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01456008 IQE jtl: Alcoholism: Clinical & Experimental Research issn: 01456008 maglogo: Y pubinfo: dt: May2021 vid: 45 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 150368893 149609337 150368893 150368893 10.1111/acer.14589 150368893 ppf: 934 ppct: 14 formats: tig: atl: Alcohol‐associated intestinal dysbiosis alters mucosal‐associated invariant T‐cell phenotype and function. aug: au: Gu, Min Samuelson, Derrick R. Taylor, Christopher M. Molina, Patricia E. Luo, Meng Siggins, Robert W. Shellito, Judd E. Welsh, David A. affil: Section of Pulmonary/Critical Care and Allergy/Immunology, Department of Internal Medicine, Louisiana State University Health Science Center, New Orleans LA,, USA sug: subj: Ethanol Adverse Effects Intestinal Mucosa Pathology Pathologic Processes T Lymphocytes Metabolism T Lymphocytes Physiology Phenotype Immune System Physiopathology Animal Studies Mice Ethanol Administration and Dosage Flow Cytometry Gut Microbiota Cecum Microbiology Antibiotics Therapeutic Use Lung Physiopathology Liver Physiopathology Gene Expression Transcription Factors Fecal Microbiota Transplantation ab: Background: Chronic alcohol consumption is associated with a compromised innate and adaptive immune responses to infectious disease. Mucosa‐associated invariant T (MAIT) cells play a critical role in antibacterial host defense. However, whether alcohol‐associated deficits in innate and adaptive immune responses are mediated by alterations in MAIT cells remains unclear. Methods: To investigate the impact of alcohol on MAIT cells, mice were treated with binge‐on‐chronic alcohol for 10 days and sacrificed at day 11. MAIT cells in the barrier organs (lung, liver, and intestine) were characterized by flow cytometry. Two additional sets of animals were used to examine the involvement of gut microbiota on alcohol‐induced MAIT cell changes: (1) Cecal microbiota from alcohol‐fed (AF) mice were adoptive transferred into antibiotic‐pretreated mice and (2) AF mice were treated with antibiotics during the experiment. MAIT cells in the barrier organs were measured via flow cytometry. Results: Binge‐on‐chronic alcohol feeding led to a significant reduction in the abundance of MAIT cells in the barrier tissues. However, CD69 expression on tissue‐associated MAIT cells was increased in AF mice compared with pair‐fed (PF) mice. The expression of Th1 cytokines and the corresponding transcriptional factor was tissue specific, showing downregulation in the intestine and increases in the lung and liver in AF animals. Transplantation of fecal microbiota from AF mice resulted in a MAIT cell profile aligned to that of AF mouse donor. Antibiotic treatment abolished the MAIT cell differences between AF and PF animals. Conclusion: MAIT cells in the intestine, liver, and lung are perturbed by alcohol use and these changes are partially attributable to alcohol‐associated dysbiosis. MAIT cell dysfunction may contribute to alcohol‐induced innate and adaptive immunity and consequently end‐organ pathophysiology. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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