Identification of key genes and pathways in discoid lupus skin via bioinformatics analysis.
Abstract: Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To ide...
| Publicado en: | Medicine Vol. 100; no. 16; pp. 1 - 7 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
4/23/2021
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=150503112&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 150503112 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00257974 2S6 jtl: Medicine issn: 00257974 maglogo: N pubinfo: dt: 4/23/2021 vid: 100 iid: 16 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 150503112 150503112 NLM33879674 150503112 10.1097/MD.0000000000025433 NLM33879674 150503112 ppf: 1 ppct: 6 formats: tig: atl: Identification of key genes and pathways in discoid lupus skin via bioinformatics analysis. aug: au: Qian Dong Kang Chen Jinye Xie Hui Han Yanping Feng Jianqiang Lu Weijia Wang Dong, Qian Chen, Kang Xie, Jinye Han, Hui Feng, Yanping Lu, Jianqiang Wang, Weijia affil: Department of Laboratory Medicine, Zhongshan Hospital of Sun Yat-sen University, Zhongshan, Guangdong, China sug: subj: Molecular Structure Lupus Erythematosus, Cutaneous Receptors, Cell Surface Metabolism Software Metabolic Networks and Pathways Lupus Erythematosus, Cutaneous Epidemiology Skin Signal Transduction Immunology Lupus Erythematosus, Cutaneous Pathology Lupus Erythematosus, Cutaneous Immunology Gene Expression Profiling Skin Pathology Biochemical Phenomena Signal Transduction Cytokines Oligonucleotide Array Sequence Analysis Data Collection Proteins Molecular Probe Techniques Bioinformatics Funding Source Human ab: Abstract: Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To identify candidate genes involved in the occurrence and development of the disease, we downloaded the microarray datasets GSE52471 and GSE72535 from the Gene Expression Database (GEO). DEGs between discoid lupus skin and normal controls were selected using the GEO2R tool and Venn diagram software (http://bioinformatics.psb.ugent.be/webtools/Venn/). The Database for Annotation, Visualization, and Integrated Discovery (DAVID), Enrichr, and Cytoscape ClueGo were used to analyze the Kyoto Encyclopedia of Gene and Genome pathways and gene ontology. Protein-protein interactions (PPIs) of these DEGs were further assessed using the Search Tool for the Retrieval Interacting Genes version 10.0.Seventy three DEGs were co-expressed in both datasets. DEGs were predominantly upregulated in receptor signaling pathways of the immune response. In the PPI network, 69 upregulated genes were selected. Furthermore, 4 genes (CXCL10, ISG15, IFIH1, and IRF7) were found to be significantly upregulated in the RIG-I-like receptor signaling pathway, from analysis of Enrichr and Cytoscape ClueGo.The results of this study may provide new insights into the potential molecular mechanisms of DLE. However, further experimentation is required to confirm these findings. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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